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1.40 na objective

Manufactured by Olympus
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The Olympus 100× 1.40 NA objective is a high-numerical aperture objective lens designed for microscopy applications. It provides a 100× magnification and a numerical aperture of 1.40, which allows for the collection of a large amount of light and the observation of fine details in specimens.

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Lab products found in correlation

C4742 95, by Hamamatsu Photonics (2 mentions) γh2ax, by Merck Group (2 mentions) Axioplan 2 microscope, by Hamamatsu Photonics (2 mentions) Rad51, by Merck Group (2 mentions) Sc 137021, by Santa Cruz Biotechnology (2 mentions) Dv elite cmos camera, by Olympus (2 mentions) Planapo 60 1.42 na objective, by Olympus (2 mentions)

Spelling variants of this product

UPlanSApo 100×/1.40 NA oil immersion objective (4 citations) PlanApo 100× NA 1.40 oil objective (4 citations) UApo 40×/1.35 NA oil immersion objective (3 citations) UPlanSApo 100× NA 1.40 oil objective lens (2 citations) UPlanSApo 100 ×/1.40 NA oil-immersion objective lens (2 citations) PlanApo 60× 1.40 NA objective (2 citations) UPlan-FLN 40 NA 1.3 objective (2 citations) PlanApoN × 60/1.40 NA Oil Sc objective lens (2 citations) 100× (1.40 NA) oil objective (2 citations)

2 protocols using 1.40 na objective

1

Immunofluorescence Imaging of DNA Repair Factors

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Previously published procedures were followed for IF and IF-FISH12 (link). IF for myc-tagged RPA32 or Ctc1 (mouse monoclonal, 9B11 or rabbit monoclonal, 71D10, Cell Signaling Technology), HA-tagged Stn1 (3724, Cell Signaling Technology), endogenous Polα (sc-137021, Santa Cruz), and 53BP1 (612522, BD Biosciences) was carried out using the cytoskeleton extraction protocol14 (link). Intensity measurements of RPA32-myc IF were performed in FIJI as follows: nuclei were identified using thresholding, segmented, and identified as regions of interest. The average image background was then subtracted from the image, and the total raw pixel intensity within each area of interest in the channel of interest was calculated. Rad51 (70-001, Bioacademia), and γH2AX (05636, Millipore) were detected in cells fixed in 3% PFA, and foci showing co-localization of Rad51 with γH2AX were quantified. IF imaging was performed on a Zeiss Axioplan II microscope equipped with a Hamamatsu C4742-95 camera using Volocity software or on a DeltaVision (Applied Precision) equipped with a cooled charge-coupled device camera (DV Elite CMOS Camera), a PlanApo 60× 1.42 NA objective or 100× 1.40 NA objective (Olympus America, Inc.), and SoftWoRx software.
Mirman Z., Lottersberger F., Takai H., Kibe T., Gong Y., Takai K., Bianchi A., Zimmermann M., Durocher D, & de Lange T. (2018). 53BP1/Rif1/Shieldin counteract DSB resection through CST/Polα-dependent fill-in. Nature, 560(7716), 112-116.
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2

Immunofluorescence Imaging of DNA Repair Factors

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Previously published procedures were followed for IF and IF-FISH12 (link). IF for myc-tagged RPA32 or Ctc1 (mouse monoclonal, 9B11 or rabbit monoclonal, 71D10, Cell Signaling Technology), HA-tagged Stn1 (3724, Cell Signaling Technology), endogenous Polα (sc-137021, Santa Cruz), and 53BP1 (612522, BD Biosciences) was carried out using the cytoskeleton extraction protocol14 (link). Intensity measurements of RPA32-myc IF were performed in FIJI as follows: nuclei were identified using thresholding, segmented, and identified as regions of interest. The average image background was then subtracted from the image, and the total raw pixel intensity within each area of interest in the channel of interest was calculated. Rad51 (70-001, Bioacademia), and γH2AX (05636, Millipore) were detected in cells fixed in 3% PFA, and foci showing co-localization of Rad51 with γH2AX were quantified. IF imaging was performed on a Zeiss Axioplan II microscope equipped with a Hamamatsu C4742-95 camera using Volocity software or on a DeltaVision (Applied Precision) equipped with a cooled charge-coupled device camera (DV Elite CMOS Camera), a PlanApo 60× 1.42 NA objective or 100× 1.40 NA objective (Olympus America, Inc.), and SoftWoRx software.
Mirman Z., Lottersberger F., Takai H., Kibe T., Gong Y., Takai K., Bianchi A., Zimmermann M., Durocher D, & de Lange T. (2018). 53BP1/Rif1/Shieldin counteract DSB resection through CST/Polα-dependent fill-in. Nature, 560(7716), 112-116.
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