Cp690550

Recombinant mouse IL-10 was purchased from R&D Systems. LY294002 and rapamycin were purchased from Calbiochem (EMD Millipore). CP-690550 and STO-609 were purchased from Tocris Biosciences. Western blot detection of specific proteins used the following primary Abs: anti-phospho-AMPKα (Thr172), anti-AMPKα, anti-phospho-PI3 kinase p85 (Tyr458)/p55 (Tyr199), anti-PI3 kinase p55, anti-PI3 kinase p85, anti-phospho-Akt (Ser473), anti-phospho-Akt (Thr308), anti-Akt, anti-phospho-mTOR (Ser2448), anti-mTOR, anti-phospho-p70 S6K (Ser371), anti-p70 S6K, anti-phospho-TSC2 (Ser939), anti-phospho-TSC2 (Ser1387), anti-TSC2, anti-phospho-Stat3 (Tyr705), anti-phospho-Stat3 (Ser727), anti-Stat3, anti-phospho-JAK1 (Tyr1022/1023), anti-JAK1, anti-SOCS3, anti-phospho-NF-κB (Ser536), anti-NF-κB, anti-IκB (Cell Signaling Technology), anti-β-actin (Sigma-Aldrich), and HRP-conjugated secondary Ab (Jackson ImmunoResearch Laboratories).

Deuterated and non-deuterated LM standards for ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) quantification were purchased from Cayman Chemical/Biomol GmbH (Hamburg, Germany). Archazolid A was isolated from Archangium gephyra as previously described (17 (link)). Bafilomycin A1 was obtained from Sigma-Aldrich (Taufkirchen, Germany), CP-690,550 and GSK-2033 were obtained from Tocris Bioscience (Bristol, UK), JQ-1 was from AdooQ Bioscience (Irvine, CA, USA), LY294002 was from Cytoskeleton (Denver, CO, USA), skepinone-L and Torin 1 were from Cayman chemical (Ann Arbor, MI, USA), and U0126 was from Enzo Life Sciences (Farmingdale, New York, USA). AS1517499 and all other reagents were obtained from Sigma-Aldrich (Taufkirchen, Germany) unless mentioned otherwise.