Viral RNA extraction kits (QIAamp Viral RNA minikits; Qiagen, Hilden, Germany) (Cat NO. 52906) were purchased from Qiagen (Beijing, China). Universal isothermal amplification kits, AMV Reverse Transcriptase, colorimetric indicator (malachite green, MG), and biotin-14-dCTP were obtained from Bei-Jing HaiTaiZhengYuan. Co., Ltd. (Beijing, China). The LFB materials, including the backing card, sample pad, absorbent pad, conjugate pad, and nitrocellulose membrane (NC), were purchased from Jie-Yi Biotechnology. Co., Ltd. (Shanghai, China). Anti-FAM (rabbit anti-fluorescein antibody) and biotin-BSA (biotinylated bovine serum albumin) were purchased from Abcam. Co., Ltd. (Shanghai, China). Dye (Crimson red) streptavidin-coated polymer nanoparticles (129 nm, 10 mg ml-1; 100 mM borate, pH 8.5, with 0.1% BSA, 0.05% Tween 20 and 10 mM EDTA) were purchased from Bangs Laboratories, Inc. (Indiana, USA).
Dye crimson red streptavidin coated polymer nanoparticles
Manufactured by Bangs Laboratories
Sourced in United States
Dye (Crimson red) streptavidin-coated polymer nanoparticles are a type of lab equipment produced by Bangs Laboratories. These nanoparticles have a core function of providing a fluorescent dye label with a crimson red color. The nanoparticles are coated with streptavidin, a protein that can bind to biotinylated molecules.
Automatically generated - may contain errors
Lab products found in correlation
Biotin bsa, by Abcam (8 mentions)
Qiaamp dna mini kit, by Qiagen (6 mentions)
Anti fitc, by Abcam (4 mentions)
Biotinylated bovine serum albumin, by Abcam (3 mentions)
Biotinylated bovine serum albumin biotin bsa, by Abcam (2 mentions)
Rabbit anti fluorescein antibody anti fam, by Abcam (2 mentions)
Qiaamp viral rna mini kit, by Qiagen (1 mentions)
Rabbit anti fluorescein antibody anti fitc ab, by Abcam (1 mentions)
Anti dig, by Abcam (1 mentions)
Anti fam, by Abcam (1 mentions)
10 protocols using dye crimson red streptavidin coated polymer nanoparticles
1
Viral RNA Extraction and Isothermal Amplification
2
Rapid LAMP Detection via Lateral Flow
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To achieve the rapid and simple LAMP test, we used a lateral flow biosensor (LFB) for a simple and objective report of the LAMP results, termed LAMP-LFB. Briefly, the LAMP products were loaded onto the nitrocellulose filter membrane of the LFB and the results were displayed with red lines. The test line is indicated by TL, and CL indicates the control line. The LFB materials, including sample pad, nitrocellulose membrane, conjugate pad, absorbent pad, and backing card were purchased from the Jie-Yi Biotechnology. Co., Ltd. (Shanghai, China). Colorimetric indicator (Malachite Green), universal isothermal amplification kits and biotin-14-dCTP were purchased from Bei-Jing Hai Tai Zheng Yuan. Co., Ltd. (Beijing, China). Dye (crimson red) streptavidin-coated polymer nanoparticles (129 nm, 10 mg mL−1, 100 mM borate, pH 8.5 with 0.1% bovine serum albumin, 0.05% Tween 20, and 10 mM EDTA) were purchased from Bangs Laboratories, Inc. (Fishers, IN). Anti–fluorescein isothiocyanate (rabbit antifluorescein antibody) and biotin BSA (biotinylated bovine serum albumin) were purchased from Abcam Co., Ltd. (Shanghai, China).
3
Rapid Isothermal Amplification and Visual Detection
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We got genomic template extraction kits (QIAamp DNA minikits; Qiagen, Hilden, Germany) from Qiagen (Beijing, China). Universal isothermal amplification kits and visual detection reagent (VDR) were obtained from Bei-Jing HaiTaiZhengYuan Co., Ltd. (Beijing, China). LFB materials, such as conjugate pad, sample pad, backing card, nitrocellulose membrane (NC) and absorbent pad were obtained from the Jie-Yi Biotechnology Co., Ltd. (Shanghai, China). Biotin-BSA (biotinylated bovine serum albumin) and anti-FITC (rabbit anti-fluorescein antibody), anti-dig (sheep anti-digoxigenin antibody) were purchased from Abcam Co., Ltd. (Shanghai, China). Dye (Crimson red) streptavidin-coated polymer nanoparticles (129 nm, 10 mg ml−1, 100 mM borate, pH 8.5 with 0.1% BSA, 0.05% Tween 20 and 10 mM EDTA) were obtained from Bangs Laboratories, Inc. (Indiana, USA).
4
Bacterial DNA Extraction and LFB Assay
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Bacterial genomic DNA extraction kits (QIAamp DNA minikits; Qiagen, Hilden, Germany) were purchased from Qiagen (Beijing, China). Universal isothermal amplification kits, a colorimetric indicator (malachite green, MG), and biotin-14-dCTP were obtained from Bei-Jing HaiTaiZhengYuan. Co., Ltd. (Beijing, China). The LFB materials, including the backing card, sample pad, absorbent pad, conjugate pad and nitrocellulose membrane (NC), were purchased from Jie-Yi Biotechnology. Co., Ltd. (Shanghai, China). Anti-FITC (rabbit anti-fluorescein antibody) and biotin-BSA (biotinylated bovine serum albumin) were purchased from Abcam. Co., Ltd. (Shanghai, China). Dye (Crimson red) streptavidin-coated polymer nanoparticles (129 nm, 10 mg ml− 1; 100 mM borate, pH 8.5, with 0.1% BSA, 0.05% Tween 20 and 10 mM EDTA) were purchased from Bangs Laboratories, Inc. (Indiana, USA).
5
Rapid Nucleic Acid Detection Kit
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Fungal and Bacterial genomic DNA extraction kits (Baitaike DNA mini kits, China) were obtained from Baitaike. Co., Ltd (Beijing, China). DNA Isothermal amplification kits, colorimetric indicator (Visual detection reagent, VDR), and biotin-14-dCTP were supplied by Tian-Jin HuiDeXin Biotech. Co., Ltd (Tianjin, China). The LFB materials, including the backing card, sample pad, conjugate pad, absorbent pad, and nitrocellulose membrane (NC), were obtained from Jie-Yi Biotech. Co., Ltd (Shanghai, China). Rabbit anti-fluorescein antibody (Anti-FITC) and biotinylated bovine serum albumin (biotin-BSA) were acquired from Abcam. Co., Ltd (Shanghai, China). Dye (Crimson red) streptavidin-coated polymer nanoparticles (129 nm, 10 mg ml−1; 100 mm borate, pH 8.5, with 0.1% BSA, 0.05% Tween 20 and 10 mm Ethylene Diamine Tetraacetic Acid, EDTA) were purchased from Bangs Laboratories, Inc (Indiana, United States).
6
Isothermal Amplification and Visual Detection Assay
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Absorbent pads, nitrocellulose (NC) membranes, sample pads, conjugate pads and membrane backing materials were purchased from the Jie Yi Biotechnology Co., Ltd. (Shanghai, China). Isothermal amplification kits and visual detection reagent (VDR) were purchased from BeiJing-HaiTaiZhengYuan Technology Co., Ltd. (Beijing, China). Biotinylated bovine serum albumin (biotin-BSA) and rabbit anti-fluorescein antibody (anti-FITC Ab) were purchased from the Abcam Co., Ltd. (Shanghai, China). Dye (Crimson red) streptavidin coated polymer nanoparticles (129 nm, 10 mg mL− 1, 100 mM borate, pH 8.5 with 0.1% BSA, 0.05% Tween 20, and 10 mM EDTA) were purchased from Bangs Laboratories, INC. (Indiana, USA). Genomic template extraction kits (QIAamp DNA mini kits; Qiagen, Hilden, Germany) was purchased from Qiagen Co., Ltd. (Beijing, China).
7
Sensitive Genomic Template Extraction
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Genomic template extraction kits (QIAamp DNA minikits; Qiagen, Hilden, Germany) were purchased from Qiagen (Beijing, China). Dye (Crimson red) streptavidin-coated polymer nanoparticles (129 nm, 10 mg mL-1, 100 mM borate, pH 8.5 with 0.1% BSA, 0.05% Tween 20 and 10 mM EDTA) were purchased from Bangs Laboratories, (Indiana, USA). Biotin-BSA (biotinylated bovine serum albumin) and anti-FITC (rabbit anti-fluorescein antibody), anti-dig (sheep anti-digoxigenin antibody) were purchased from Abcam (Shanghai, China). Universal isothermal amplification kits and visual detection reagent (VDR) were purchased from HaiTaiZhengYuan (Beijing, China). LFB materials, such as conjugate pad, sample pad, backing card, nitrocellulose membrane (NC) and absorbent pad were purchased from the Jie-Yi Biotechnology (Shanghai, China).
8
Rapid Genomic DNA Extraction and Colorimetric Detection
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Genomic DNA of each isolate was extracted with the DNA extraction kits (QIAamp DNA mini kits; Qiagen, Hilden, Germany). Color indicator (Visual detection reagent, VDR) and DNA isothermal amplification kit were purchased from Tian-jing Huidexing. Co., Ltd. (Beijing, China). The materials, including backing card, sample pad, conjugate pad, nitrocellulose membrane (NC) and absorbent pad, were obtained from the Jie-Yi Biotechnology. Co., Ltd. (Shanghai, China). Biotin-BSA (Biotinylated bovine serum albumin) and anti-FITC (rabbit anti-fluorescein antibody) were obtained from Abcam. Co., Ltd. (Shanghai, China). Dye (Crimson red) streptavidin-coated polymer nanoparticles (129 nm, 10 mg mL−1, 100 mM borate, pH 8.5 with 0.1% BSA, 0.05% Tween 20 and 10 mM EDTA) were purchased from Bangs Laboratories, INC. (Indiana, USA).
9
Isothermal Amplification-based Lateral Flow Biosensor
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The kit Qiagen QIAamp DNA mini kit (Qiagen, Beijing, China) was used to extract genomic DNA from fungi, bacterial and clinical. Isothermal amplification kits and colorimetric indicator (malachite green, MG) were provided by Beijing-Hai Tai Zheng Yuan Technology Co., Ltd. (Beijing, China). Dye streptavidin-coated polymer nanoparticles (Crimson red) were obtained from Bangs Laboratories, Inc. (Indiana, United States). Anti-FAM (rabbit anti-fluorescein antibody) and biotin-BSA (biotinylated bovine serum albumin) were obtained from Abcam. Co., Ltd. (Shanghai, China). Polymer nanoparticle-based lateral flow biosensor (LFB) materials, including sample pad, nitrocellulose membrane (NC), absorbent pad, conjugate pad and backing card were obtained from Jie-Yi Biotechnology. Co., Ltd. (Shanghai, China). Primers and labeled primers (Table 2 and Supplementary Table 1 ) used were synthesized by Beijing-Tsingke Biotechnology Co., Ltd. (Beijing, China).
10
Rapid Isothermal Nucleic Acid Detection Kit
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Genomic DNA/RNA nucleic acid extraction kits were obtained from Tianlong Technology Co., Ltd (Xian, China). Colorimetric indicator (Malachite Green, MG) and universal isothermal amplification kits were purchased from Bei-Jing HaiTaiZhengYuan. Co., Ltd. (Beijing, China). Biotin-BSA (biotinylated bovine serum albumin) and Anti-FAM (rabbit anti-fluorescein antibody) were obtained from Abcam. Co., Ltd. (Shanghai, China). Dye streptavidin-coated polymer nanoparticles (Crimson red) were obtained from Bangs Laboratories, Inc. (Indiana, USA). Polymer nanoparticle-based lateral flow biosensor (LFB) materials, including sample pad, conjugate pad, absorbent pad, nitrocellulose membrane (NC) and backing card were obtained from Jie-Yi Biotechnology. Co., Ltd. (Shanghai, China). HBV qPCR diagnosis kits were purchased from Da An Gene Co., Ltd. (Guangzhou, China). The purity and concentration of nucleic acid was analyzed with Nano-Drop ND-2000 (Beijing, China) at A260/280.
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