Hydrogen peroxide solution

Hydride chloride (37 weight percent, wt%), ethanol, nitromethane, sodium hydroxide, glacial acetic acid, hydrogen peroxide solution (30 wt%), L-ascorbic acid (AA), uric acid (UA), L-cysteine (Cys), and glutathione (GSH) were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China, www.en.reagent.com.cn). Sodium acetate anhydrous was purchased from Sigma-Aldrich Chemical Co., Ltd. (St. Louis, MO, USA, www.sigmaaldrich.com/united-states.html). 2-(tert-butoxycarbonyl)-2-methyl-3,4-dihydro-2H-pyrrole 1-oxide (BMPO) was purchased from ApexBio Technology. (7505 Fannin street, Suite 410, Houston, USA, www.apexbt.com). Pd(CH₃CN)₂Cl₂, ammonium hydroxide solution (25–28 wt%), 3,3′,5,5′-tetramethylbenzidine (TMB), silicon (IV) oxide, ammonium persulfate (APS), aniline, dimethyl sulfoxide (DMSO), potassium thiocyanate (KSCN), (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid and DL-homocysteine (Hcy) were purchased from Shanghai Titan Scientific Co., Ltd. (Shanghai, China, www.tansoole.com). All chemicals were used as received without any further purification.

Star anise fruits and black pepper fruits were bought from Jiangda South Road Vegetable Market, Nanchang City, China. Magnesium sulfate (MgSO₄), potassium dichromate (K₂Cr₂O₇), sulfonic type solid acid, potassium permanganate (KMnO₄), hydrochloric acid (HCl), ethanol absolute, hydrogen peroxide solution (30 wt. % in H₂O, H₂O₂), Iron(III) chloride (FeCl₃), methanol, sodium carbonate (Na₂CO₃), hexane, formaldehyde, thionyl chloride (SOCl₂), ethyl acetate, sodium hydroxide (NaOH), acetone, diethyl ether, potassium hydroxide (KOH), benzene, sodium bicarbonate (NaHCO₃), N,N-Dimethylformamide (DMF), 1,1-diphenyl-2-picryhydrazyl (DPPH), and butylated hydroxytoluene (BHT) were obtained from Sinopharm Chemical Reagent Co., Ltd.
The microorganisms Staphylococcus aureus (ATCC 33591) (MRSA), Streptococcus pyogenes (ATCC 19615), Escherichia coli (ATCC 9637), Salmonella typhi (ATCC 14028) and Pseudomonas aeruginosa (ATCC 10145) were purchased from Jinuo Biomedical Technology Co., Ltd (Hangzhou, China).
MTT [3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide] and C6 cells were bought from Cell Bank of Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (Shanghai, China).

h-BN powder (1 μm, 98%) was purchased from Sigma-Aldrich. N,N-dimethylformamide (DMF), di-tert-butylperoxide. and acetonitrile of analytical grade were obtained from Aladdin Reagent Limited Corporation. Sulfuric acid (98%), hydrochloric acid (36~38%), hydrogen peroxide solution (28%), and toluene were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai China). Polyvinyl alcohol (PVA) and glutaraldehyde (GA, 50% aqueous solution) were supplied by Tansoole (Shanghai, China).

The ovary tissues were washed with PBS and soaked in 4% paraformaldehyde for more than 24 h, dehydrated with ethanol, and embedded into paraffin blocks. The paraffin blocks were cooled in the freezer, sliced into 4 μm thick slices, and mounted on adhesion-treated slides. After deparaffinization, xylene was removed with a graded series of alcohols. Next, the slides were placed into a citric acid buffer (PH 6.0) for antigen retrieval in a microwave and endogenous peroxidase was blocked by a 3% hydrogen peroxide solution (Sinopharm, Beijing, China, Cat#10011208). After blocking with 3% BSA to decrease background staining, the treatment sections were incubated with primary antibodies against IL-11 and IL-11Rα (Table S1) and the negative controls were incubated with 3% BSA overnight at 4 °C. After three washes in PBS, the sections were incubated with the appropriate secondary antibodies (Table S1) for 50 min at room temperature and then stained with 3,3′-diaminobenzidine (DAB) solution (Servicebio, Wuhan, China, Cat#G1212). Subsequently, the sections were restained with hematoxylin (Servicebio, Wuhan, China, Cat#G1004) for 3 min and then dehydrated and sealed. Images were observed and captured under a microscope (Nikon, Tokyo, Japan).