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Anti human cd3 bv786

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Anti-human CD3-BV786 is a fluorochrome-conjugated antibody that specifically binds to the CD3 antigen expressed on human T cells. It can be used for the identification and enumeration of T cells in flow cytometry applications.

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Lab products found in correlation

2b4 fitc, by BD (1 mentions) Cd38 bv421, by BioLegend (1 mentions) Tigit pe cy7, by Thermo Fisher Scientific (1 mentions) Cd45ra af700, by BD (1 mentions) Cd8 bv510, by BD (1 mentions) Cd70 pe, by BD (1 mentions) Cd160 af488, by BD (1 mentions) Tim 3 bv650, by BD (1 mentions) Cd28 bv711, by BioLegend (1 mentions) Cd27 bv650, by BioLegend (1 mentions) Lag 3 apc, by Thermo Fisher Scientific (1 mentions) Cd4 apc fire750, by BD (1 mentions) Hla dr af700, by BioLegend (1 mentions) Cd95 pecy7, by BD (1 mentions) Ccr7 bv421, by BioLegend (1 mentions) Pd 1 bv711, by BD (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) Anti human cd69 bv605, by BioLegend (1 mentions) Percp anti human cd3, by BioLegend (1 mentions) Fix perm kit, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Anti-CD3 (541 citations) Anti-human CD3 (37 citations) CD3-BV786 (19 citations) Anti-CD3 BV786 (2 citations)

3 protocols using anti human cd3 bv786

1

Multiparametric Flow Cytometry of PBMCs

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PBMCs were incubated with directly conjugated antibodies for 30 min at 4°C. The cells were washed before flow cytometry analysis. Antibodies used included anti-human CD3-BV786, CD4-APC-Fire750, CD8-BV510, CD45RA-AF700, CD70-PE, PD-1-BV711, 2B4-FITC, CD160-AF488, TIM-3-BV650, CD95-PE-CY7 (BD Biosciences, San Diego, CA, USA), CCR7-BV421, HLA-DR-AF700, CD38-BV421, CD28-BV711, CD27-BV650 (BioLegend, San Diego, CA, USA), TIGIT-PE-Cy7, LAG-3-APC (Ebioscience, San Diego, CA, USA) and the corresponding isotype controls. Data acquisition was performed on an LSR Fortessa flow cytometer (BD Biosciences), and data was analyzed with FlowJo software (Tree Star, Ashland, OR, USA).
Wang D., Du J., Song Y., Wang B., Song R., Hao Y., Zeng Y., Xiao J., Zheng H., Zeng H., Zhao H, & Kong Y. (2020). CD70 contributes to age-associated T cell defects and overwhelming inflammatory responses. Aging (Albany NY), 12(12), 12032-12050.
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2

Multicolor Flow Cytometry for Characterizing Immune Cells

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Anti-human CD3 PerCP (Clone: UCHT1), anti-human CD4 BV650 (Clone: RPA-T4), anti-human TCR V7.2 AF647 (Clone: 3C10), anti-human CD161 PE/Dazzle 594 (Clone: HP-3G10) and BV785 (Clone: HP-3G10), anti-human CD69 BV605 (Clone: FN50), anti-human CD8 APC/Fire 750 (Clone: SK1), anti-human TNF-α PE/Cyanine7 (clone: MAb1), anti-human IFN-γ AF488 (Clone: 4S.B3), anti-human perforin BV421 (Clone: DG9) and anti-human/mouse granzyme B PE (Clone: QA16A02) were from BioLegend Inc. Anti-human CD3 BV786 (Clone: SK7) was from BD Biosciences.
Hubrack S., Al-Nesf M.A., Agrebi N., Raynaud C., Khattab M.A., Thomas M., Ibrahim T., Taha S., Dermime S., Merhi M., Kulinski M., Steinhoff M., Tang P, & Lo B. (2021). In vitro Interleukin-7 treatment partially rescues MAIT cell dysfunction caused by SARS-CoV-2 infection. Scientific Reports, 11, 14090.
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3

Flow Cytometry Analysis of T-Cell Subsets

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After restimulation, cells were washed twice in PBS (GIBCO). To exclude dead cells, Zombie-Dye Aqua (BioLegend) was added. Cells were washed, stained with anti-human CD3-BV786 (BD Biosciences) for 15 min, and incubated with the Fix&Perm kit (Invitrogen, Life Technologies, Grand Island, USA). Next, cells were stained with anti-human IL-17-APC-eFluor780
(eBioscience) and IFN-BUV395 (BD Biosciences) to determine the frequencies of Th17-(IL-17producing) and Th1 (IFN-producing) cells [10, 11] . Samples were acquired on a LSR-II and analysed in Kaluza v1.7 (Beckman Coulter, Brea, CA, USA). For representative gating examples, see Fig. 2A.
von Borstel A., Lintermans L.L., Heeringa P., Rutgers A., Stegeman C.A., Sanders J.S, & Abdulahad W.H. (2019). Circulating CD24hiCD38hi regulatory B cells correlate inversely with the ThEM17 cell frequency in granulomatosis with polyangiitis patients. Rheumatology (Oxford, England).
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