The Arl8a/Arl8b antibody was a generous gift from Dr. Michael Brenner (Harvard) and was used at a 1:500 dilution for immunoblot [32 (link)]. The Zeb1 antibody (Cell Signaling Technologies, Beverly, MA) was used at 1:500. The LAMP-1 (H4A3) antibody was used at 1:200 (Developmental Studies Hybridoma Bank at Iowa State University). Transforming growth factor beta (Sigma Aldrich, St. Louis, MO) was used at 5 ng/mL. Anti-tubulin was acquired from NeoMarkers (Fremont, CA) and used at 1:20,000. Anti-actin was obtained from Sigma-Aldrich and used at 1:5,000. E-cadherin and cathepsin B antibodies (Santa Cruz Biotechnology, Dallas, TX) were used at 1:1,000. The cathepsin D antibody was obtained from Calbiochem (Billerica, MA) and used at 1:1,000. NHE1, vimentin, cathepsin L, and β-catenin antibodies were purchased from BD Biosciences (San Jose, CA) and were used at 1:1,000. Secondary antibodies include Dylight 594 donkey anti-mouse (1:200) (Jackson ImmunoResearch, West Grove, PA) and HRP-conjugated anti-mouse and anti-rabbit (1:5,000) (GE Healthcare, Pittsburgh, PA). Phalloidin 488 and 633 (Invitrogen, Carlsbad, CA) were used at 1:200. Primary antibodies used for ChIP were purchased from Santa Cruz Biotechnology: Mouse IgG (CS-2025) and Zeb1 (H-102).
Transforming growth factor beta
Manufactured by Merck Group
Sourced in Belgium
Transforming growth factor beta is a protein that regulates cell growth and differentiation. It is a commonly used lab equipment for biological research.
Automatically generated - may contain errors
Lab products found in correlation
Anti actin, by Merck Group (1 mentions)
Vimentin, by BD (1 mentions)
β catenin, by BD (1 mentions)
Zeb1 h 102, by Santa Cruz Biotechnology (1 mentions)
Zeb1 antibody, by Cell Signaling Technology (1 mentions)
Dylight 594 donkey anti mouse, by Jackson ImmunoResearch (1 mentions)
Thrombin, by Merck Group (1 mentions)
Tgf β, by Merck Group (1 mentions)
Interleukin 6 (il 6), by R&D Systems (1 mentions)
2 protocols using transforming growth factor beta
1
Antibody Use in Western Blot and ChIP Assays
2
Endothelial Cell Response to Inflammatory Modulators
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were cultured for 6 h in either the absence or presence of inflammatory modulators, after which the supernatant was collected for the measurement of ET-1 and frozen at -80°C. A number of pro-inflammatory and anti-inflammatory cytokines were tested: TNF-α (Tumor Necrosis Factor alfa; Miltenyi Biotec, The Netherlands) at concentrations of 1, 10, 50, 100, and 250 ng/ml, IFN-γ (Interferon gamma; Life Technologies, Belgium) at a concentration of 100 ng/ml, IL-1β (Interleukin -1 beta; Life Technologies, Belgium) at 1, 10, 50, 100, and 250 ng/ml, LPS (Lipopolysacharide; Sigma Aldrich, Germany) at 0.5 and 10 μg/ml, thrombin (Sigma Aldrich, Germany) at 3 units/ml, IL6 (interleukin 6; R&D systems, Germany) at 10 ng/ml and 100 ng/ml, IL-10 (interleukin-10; Life Technologies, Belgium) at 10ng/ml and 100ng/ml and TGF-β (Transforming Growth Factor -beta; Sigma Aldrich, Belgium) at 10 ng/ml and 100 ng/ml. TNF-α, IL-1β, IL6 and IL10 were solved in LPS-free water (pharmacy University Hospital Brussels); LPS in PBS; thrombin in Ultrapure Water (Sartorius Biotech, type: Arium® Pro UV, Germany); IFN-γ in BSA (N,O-bis (trimethylsilyl) acetamide, Sigma Aldrich, Germany). The concentrations of the cytokines used were chosen according to previous experiments in cell cultures reported in the literature.
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