Las 4
The Leica LAS 4.3 is a software package that provides comprehensive image acquisition, processing, and analysis capabilities for a wide range of microscopy applications. It offers a user-friendly interface and advanced tools for researchers and scientists.
Lab products found in correlation
30 protocols using las 4
Imaging and Preservation of Insect Specimens
Histological Analysis of Testicular Abnormalities
Quantitative Analysis of 17β-HSD6 Immunoexpression
Quantitative Immunofluorescence Analysis of Epididymal Proteins
The measurement of AR, KPNA and SHBG immunoexpression was performed in the proximal region of four non-serial sections of the cauda epididymidis per animal. In the AR and KPNA immunolabeled sections, a standardized total epithelial area of 50,000 µm 2 was measured. In this area, the nuclear and cytoplasmic AR immunofluorescent areas as well as KPNA immunofluorescent area were measured, and the area per µm 2 of epithelium was obtained. The AR immunofluorescent nuclear/ cytoplasmic ratio was also calculated. SHBG immunofluorescent area was measured in a standardized total connective tissue area of 560,000 µm 2 per animal, and the immunofluorescent area/µm 2 of connective tissue was calculated.
During each immunoreaction analyzed in the sections from CG and CMTG, all the parameters of the software, including threshold adjustment and color range -hue, saturation and intensity (LAS 4.3, Leica) -were rigorously calibrated/ standardized for each image analyzed.
Dissection and Imaging of Cockroach Anatomy
Identification of Neophyllaphis Aphids
Liposomal Diffusion Characterization via Tracking
Larval Growth Evaluation Protocol
The same procedure was also used to sample larvae at 29 dph (intermediate sampling) and 36 dph (final sampling). At the latter sampling point, a pool of 17 larvae from each tank was also collected for biochemical analyses, frozen at −80 °C, and subsequently lyophilized until analyses could be conducted.
Furthermore, 3 different samples of enriched rotifers and Artemia sp. metanauplii were collected, frozen at −80 °C, and lyophilized until biochemical analyses. Samples of the inert diets were also stored at −80 °C until analyses. The biochemical and fatty acid composition of the diets is presented in
Immunofluorescence Imaging of Tumor Cells
Probing Mucus Dynamics: Microscopic Analysis
(Where x and y represent the coordinates of the particle, and τ represent time scale or time lag).
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