Glucose detection kit

The carrier oil is Fluorinert FC-70 (Hampton Research Corp., CA, USA). The viscosity of the carrier oil can be increased by dissolving Teflon® (AF1600, Dupont Co., DE, USA) to enhance the spatial stability of the floating droplets. The glucose detection kit, the SuperSignal® Femto-ELISA substrate, and the NSE detection kit are all from Sigma-Aldrich Corp. (Oakville, ON, USA).

Cells were plated at a density of 4.0 × 10⁵ cells/well in a six-well plate, incubated at 37℃ for 24 h to allow adherence, and then treated with different concentrations of G-Rh2 for 24 h. Subsequently, the cells were cultured in DMEM without FBS for serum starvation. The samples were assayed using a glucose detection kit (Sigma), lactate detection kit (Nanjing Jiancheng Bio), and pyruvate content detection kit (Solarbio, China). After adding the detection reagent, the optical density values were measured at 540 nm, 530 nm, and 520 nm using a microplate reader. A standard curve was generated by analyzing different standard dilutions, and the resulting OD values were utilized to determine glucose intake, lactification, and pyruvic acid production.