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Human il 1b elisa kit

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The Human IL-1B ELISA kit is a laboratory assay used to detect and quantify the levels of interleukin-1 beta (IL-1B), a pro-inflammatory cytokine, in human samples. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to measure the concentration of IL-1B in the samples.

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Lab products found in correlation

Ha tag, by Santa Cruz Biotechnology (2 mentions) Il 1b, by R&D Systems (2 mentions) Casp1, by Santa Cruz Biotechnology (2 mentions) Flag tag (flag), by Merck Group (2 mentions) Gapdh, by Santa Cruz Biotechnology (2 mentions)

2 protocols using human il 1b elisa kit

1

Antibody-based Protein Detection Protocol

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The following antibodies were used in this study: HA tag (Santa Cruz Biotechnology, #sc-805, 1:1000 for western blot), GAPDH (Santa Cruz Biotechnology, #sc-47724, 1:1000 for western blot), ASC (Adipogen, #AL-177, 1:1,000 for western blot), CASP1 (Santa Cruz Biotechnology, #sc-622, 1:1000 for western blot), IL-1B (R&D systems, #AF-201, 0.1 µg/mL for western blot, 2 µg/mL for immunochemistry), FLAG (SigmaAldrich, #F3165, 1:2000 for western blot), GFP (Abcam, #ab290, 1:2,000 for western blot). IL-1B measurements were carried out with human IL-1B ELISA kit (BD, #557953, follow supplier instructions).
Gong Q., Robinson K., Xu C., Huynh P.T., Chong K.H., Tan E.Y., Zhang J., Boo Z.Z., Teo D.E., Lay K., Zhang Y., Lim J.S., Goh W.I., Wright G., Zhong F.L., Reversade B, & Wu B. (2021). Structural basis for distinct inflammasome complex assembly by human NLRP1 and CARD8. Nature Communications, 12, 188.
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2

Antibody Characterization for Inflammasome

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The following antibodies were used in this study: HA tag (Santa Cruz Biotechnology, #sc-805), GAPDH (Santa Cruz Biotechnology, #sc-47724), ASC (Adipogen, #AL-177), CASP1 (Santa Cruz Biotechnology, #sc-622), IL1B (R&D systems, #AF-201), FLAG (SigmaAldrich, #F3165), GFP (Abcam, #ab290). IL-1B measurements were carried out with human IL-1B ELISA kit (BD, #557953).
Gong Q., Robinson K.S., Xu C., Zhang J., Zhi B.Z., Teo D.E.T., Zhang Y., Lim J.S.Y., Ing G.W., Wright G., Zhong F.L., Wu B., & Reversade B. (2020). Structural basis for distinct inflammasome complex assembly by human NLRP1 and CARD8.
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