L lactic acid colorimetric assay kit

A431 cells with or without the indicated transfection were cultured normally for 24 h to collect the supernatant. The levels of glucose, lactic acid and ATP in the supernatant were analyzed with Glucose Assay kit (cat. no. E-BC-K234-S; Elabscience), L-Lactic Acid Colorimetric Assay kit (cat. no. E-BC-K044-S; Elabscience) and ATP Assay Kit (cat. no. S0026; Beyotime).

During the cultivation, the cell culture supernatant was collected. The L-Lactic Acid (LA) Colorimetric Assay Kit (#E-BC-K044-M, Elabscience Biotechnology Co.,Ltd, China) was used to analyze the lactic acid concentrations following the manufacturer’s instructions.

The lactate concentration in the medium was assessed following the protocol provided by the l-lactic acid (LA) Colorimetric Assay Kit (Elabscience, Wuhan, China). LX-2 cells were cultured for 48 h, and the supernatants were collected and diluted with PBS. A 5 µL aliquot of the diluted sample was taken for each well, followed by the addition of 100 µL of enzyme working solution and 20 µL of chromogenic agent. The mixture was then incubated at 37 °C for 10 min. A stop solution was added to each well, and the absorbance was measured at 530 nm using a full-wavelength microplate reader. To account for variations in cell number, the lactate content was normalized using a cell counter (Nexcelom Bioscience, USA). A similar procedure was employed to determine lactate levels in serum.