Serum levels of immune-related cytokines will be measured using commercial ELISA kits for TNF-α, IFN-γ as well as IL-2, IL-10, and IL-12 (BD Biosciences, San Jose, CA, USA). Absorbance will be measured with a spectrophotometer (Molecular Devices, Sunnyvale, CA, USA).
Elisa kits for tnf α
Manufactured by BD
Sourced in United States
ELISA kits for TNF-α are laboratory products designed to detect and quantify the presence of the cytokine tumor necrosis factor-alpha (TNF-α) in biological samples. These kits utilize the enzyme-linked immunosorbent assay (ELISA) technique to measure the concentration of TNF-α in the samples.
Automatically generated - may contain errors
Lab products found in correlation
Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Il 1β, by R&D Systems (1 mentions)
Interleukin 6 (il 6), by R&D Systems (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Il 1β, by BD (1 mentions)
Cox 2, by Cell Signaling Technology (1 mentions)
Cox 1, by Cell Signaling Technology (1 mentions)
P jnk, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
P erk, by Cell Signaling Technology (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
4 protocols using elisa kits for tnf α
1
Cytokine Profiling by ELISA
2
Quantification of Serum Calprotectin and Colonic Cytokines
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Serum calprotectin levels were evaluated by performing the Mouse CALP (Calprotectin) ELISA Kit (Elabscience, USA) from mice blood as recommended by the user manual.
Cytokines were quantified from the whole colon mucosae and from culture supernatant of colonic macrophages or BMDM with ELISA kits for TNF-α, IL-1β, IL-6, TGF-β, and IL-10 (BD Biosciences, R&D Systems) according to the manufacturer’s instructions.
Cytokines were quantified from the whole colon mucosae and from culture supernatant of colonic macrophages or BMDM with ELISA kits for TNF-α, IL-1β, IL-6, TGF-β, and IL-10 (BD Biosciences, R&D Systems) according to the manufacturer’s instructions.
3
Quantifying Inflammatory Cytokines via ELISA
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According to the manufacturer’s instructions, the levels of inflammatory cytokines in serum, lung, liver, and culture media were measured using commercial enzyme-linked immunosorbent assay (ELISA) kits for TNF-α (BD Biosciences, San Jose, CA, USA), IL-6 (R&D Systems, Minneapolis, MN, USA) and IL-1β (R&D Systems, Minneapolis, MN, USA). The protein concentration was quantified with a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Lafayette, CO, USA). The ELISA results from tissue samples were normalized according to the protein content (pg/mg protein).
4
Inflammatory Signaling Pathway Analysis
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Fetal bovine serum (FBS), Dulbecco's modified Eagle's medium (DMEM), antibiotics (penicillin and streptomycin were obtained from Invitrogen-Gibco (Grand Island,NY). Dimethyl sulfoxide (DMSO) , lipopolysaccharide (LPS; from Escherichia coli strain) and phosphate buffered saline (PBS) were purchased from Sigma Chemical Co(St. Louis, MO, USA). The ELISA kits for TNF-α, PGE2, IL-1β, and IL-6 were obtained from BD Biosciences (San Diego, CA, USA) and R&D Systems, Inc. (St. Louis, MO, USA). Anti-bodies that includes: iNOS, COX-1, COX-2, IκB-α, ERK, JNK, p38, p-ERK, p-JNK, p-p38 and β-actin were purchased from Cell Signaling Technology (Beverly, MA, USA). The secondary antibodies were obtained from Cell Signaling Technology.
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