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Casein powder

Manufactured by Merck Group
Sourced in United States

Casein powder is a protein-based product derived from milk. It is a fine, white, powdery substance that serves as a key component in various laboratory processes and applications.

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4 protocols using casein powder

1

Pollen-Based Diets for Honey Bees

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Diets were prepared using a combination of pollen collected by honey bees, alpha-cellulose powder (Sigma-Aldrich, St. Louis, MO, USA), casein powder (Sigma-Aldrich, St. Louis, MO, USA), organic flaxseed oil (EarthSource), and DI water. Pollen was ground using a mortar and pestle until it was a fine powder and used as is (100%, “control diet”) or diluted with alpha cellulose to 50% of its original amount (“5:1 low” diet, Exp. 2). Depending on the intended macronutrient ratio and composition of the diet, predetermined amounts of casein powder or flaxseed oil were added (recipes are listed in Table 1). DI water was added to ensure diet texture and appearance were similar across all diets. To verify the total protein and lipid amounts, all diets were analyzed for their macronutrient composition (Table 1). Diet samples that were used for macronutrient composition did not include water.
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2

Fabrication of IGZO and ITO Thin Films

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Corning 7509 glass substrate (Corning Inc., Corning, NY, USA), casein powder derived from bovine milk (technical grade, Sigma-Aldrich, Seoul, Korea), an acetic acid solution (purity > 99%, Sigma-Aldrich), IGZO sputter target (In2O3:Ga2O3:ZnO = 4:2:4.1 mol%, THIFINE Co., Ltd., Incheon, Korea), and ITO sputter target (In2O3:SnO2 = 9:1 mol%, THIFINE Co., Ltd., Korea) were the materials used in this study.
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3

Fluorescent Kinesin-1 Construct Purification

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Unlabeled and labeled (biotin and tetramethylrhodamine (TRITC)) lyophilized tubulin from porcine brain were purchased from Cytoskeleton. Neutravidin was purchased from Life Sciences (St. Louis, MO). Casein powder, glucose oxidase from Aspergillus niger, and bovine liver catalase were purchased from Sigma (St. Louis, MO). Mouse monoclonal anti-6xHis antibody was purchased from Abcam (Cambridge, MA). Biotinylated mouse anti-5xHis antibody was purchase from QIAGEN (Hilden, Germany). Mouse anti-tubulin β3 antibody from Bio-Rad Laboratories (Hercules, CA). We used a 6xHis-GFP-labeled truncated human kinesin-1 heavy chain construct (aa 1–560 (15 (link))) with an AviTag sequence susceptible to biotinylation added at the C-terminus (16 (link)). The protein was expressed in Escherichia coli, purified using cobalt resin from Clontech Laboratories (now Takara Bio USA, Mountain View, CA) (15 (link)). Biotin ligase BirA (Avidity) was used to biotinylate AviTag-kinesin (17 (link)).
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4

Casein-Hyaluronic Acid-Vitamin E Formulation

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Casein powder (80 % protein) was obtained from Sigma-Aldrich. Hyaluronic acid (194 kDa) was obtained from Focusfreda Biological Co., ltd. (Shandong, China). Vitamin E was purchased from Shanghai Yuanye Bio-Technology Co., ltd. (Shanghai, China). Other chemicals were of analytical grade.
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