The C. militaris strain KCTC 6064 was purchased from the Wuhan Academy of Agricultural Sciences in China (Wuhan, China). Glucose, peptone, KH2PO4, MgSO4, KF, and acetone were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Rice was purchased from the National Federation of Agricultural Co-operative Associations (Ibaraki, Japan). Superoxide dismutase (SOD) Assay Kit-WST was purchased from Dojindo Molecular Technologies, Inc. (Tabaru, Kumamoto, Japan); 2,2-diphenyl-1-picrylhydrazyl (DPPH), Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), and the penicillin–streptomycin solution were purchased from Thermo Fisher Scientific K.K. (Tokyo, Japan).
Peptone
Manufactured by Fujifilm
Sourced in Japan
Peptone is a laboratory product that serves as a nutrient source for the growth and cultivation of microorganisms in microbiology and biotechnology applications. It is a complex mixture of amino acids and peptides derived from the enzymatic digestion of proteins.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Glucose, by Fujifilm (2 mentions)
Glycerol, by Fujifilm (2 mentions)
Yeast extract, by Fujifilm (2 mentions)
Methanol, by Fujifilm (2 mentions)
Acetone, by Fujifilm (1 mentions)
Kh2po4, by Fujifilm (1 mentions)
Mgso4, by Fujifilm (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)
Trehalose, by Fujifilm (1 mentions)
Casein, by Fujifilm (1 mentions)
K2hpo4, by Fujifilm (1 mentions)
Lab lemco powder, by Thermo Fisher Scientific (1 mentions)
L cysteine hcl, by Kanto Chemical (1 mentions)
Sucrose, by Fujifilm (1 mentions)
Agar, by Fujifilm (1 mentions)
Polysorbate 80, by BD (1 mentions)
Potato dextrose agar (pda), by Fujifilm (1 mentions)
5 protocols using peptone
1
Cultivation and Bioactivity of Cordyceps militaris
2
Drosophila Rearing Conditions Protocol
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Drosophila melanogaster flies were reared on standard agar-cornmeal media at 25 °C unless otherwise indicated8 (link), 50 (link). The detailed food compositions, except preservatives, are shown in Fig.4B . Casein, peptone, and trehalose were obtained from Wako Chemical, BD Biosciences, and Hayashibara, Co., respectively. All experiments were conducted under non-crowded conditions. No yeast paste was added to the fly tubes for any of the experiments. The percentage of puparium formation and adult flies was determined by counting homozygotes and heterozygotes in the same vials as an internal control.
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Drosophila melanogaster flies were reared on standard agar-cornmeal media at 25 °C unless otherwise indicated8 (link), 50 (link). The detailed food compositions, except preservatives, are shown in Fig.
3
Anaerobic Nurmi-type Culture Preparation
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VL broth or NB was used as the basal culture medium for preparing Nurmi-type cultures. VL broth contained 10 g Bacto Tryptone (BD Difco), 3 g Lab-Lemco powder (Oxoid), 5 g Bacto Yeast Extract (BD Difco), 2.5 g glucose, 5 g NaCl, 0.4 g l -cysteine HCl (Kanto Chemical Co., Inc., Tokyo, Japan), and 0.6 g agar in 1 liter of distilled water adjusted to pH 7.2 using 1 N NaOH (Barnes and Impey, 1970 ). NB contained 10 g Bonito extract (Wako Pure Chemical Industries, Ltd., Tokyo, Japan), 10 g peptone (Wako), 2 g NaCl, 5 g K2HPO4, and 0.8 g agar (Wako) in 1 liter of distilled water adjusted to pH 7.0 using a 1 N NaOH solution. Other broth cultures were prepared with or without Cys. Cys was added to NB (NB+Cys) and not to VL broth, which usually contains Cys in its original formulation (VL−Cys). The final Cys concentration in each broth was 2 mM (Barnes and Impey, 1970 ). Each Nurmi-type culture was started by inoculating 1 ml of mixed CE source into 100 ml of each medium followed by incubation at 37°C for 24 h under anaerobic conditions without agitation, using the AnaeroPak method (Mitsubishi Gas).
4
Microbiological Culture Media Preparation
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The chemicals and solvents; Ethanol, MEthanol, Glycerol, Agar, Potato Dextrose Agar, Peptone, Glucose, Sucrose, Yeast Extract, Paper disk, ODS C-18 were purchased from Wako Pure Chemical Industries Ltd. (Osaka, Japan). Fetal bovine serum (FBS) was purchased from Invitrogen (Carlsbad, CA, USA). Middlebrook 7H9 broth, polysorbate 80, and Middlebrook OADC were purchased from BD.
5
Synthesis and Characterization of PLLA-GRGDS Bioconjugate
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Peptone, yeast extract, chloroform, methanol, glycerol, 10-undecenoic acid, potassium dihydrogen phosphate (KH 2 PO 4 ), diammonium hydrogen phosphate [(NH 4 ) 2 HPO 4 ], citric acid, triethylamine, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), and magnesium sulfate heptahydrate (MgSO 4 •7H 2 O) were purchased from Wako Pure Chemical Industries (Osaka, Japan). Fluorescein isothiocyanate (FITC), chlorobenzene, and acetone were purchased from Sigma-Aldrich Japan (Tokyo, Japan). 3-Mercaptopropionic acid, 2-aminoethanthiol hydrochloride, and 2,2-dimethoxy-2-phenylacetophenon (DMPA) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). All other chemicals were of reagent grade or better. Poly-ʟ-lactic acid (PLLA) was also purchased from Sigma-Aldrich Japan. The fibronectin active fragment (Gly-Arg-Gly-Asp-Ser: GRGDS) was the product of Peptide Institute, Inc. (Osaka, Japan).
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