F, DHEA, Δ5-diol, E2, and fusaric acid (FA) were from Sigma-Aldrich (St. Louis, MO, USA). 7α-OH-DHEA and 3βAdiol were from Steraloids (Newport, RI, USA). F-d4 was from C/D/N Isotopes Inc. (Quebec, Canada). DHEA-13C3 was synthesized at Tohoku Pharmaceutical University (Miyagi, Japan). Δ5-diol-d4 and 7α-OH-DHEA-d2 were synthesized at ASKA Pharma Medical Co. Ltd. (Kanagawa, Japan). E2-13C4 was from Hayashi Pure Chemical Ind. Ltd. (Osaka, Japan). 4-dimethylaminopyridine (DMAP), 2-methyl-6-nitrobenzoic anhydride (MNBA), and picolinic acid (PA) were from Tokyo Chemical Industry (Tokyo, Japan). Triethylamine (TEA), O-ethylhydroxyl-ammonium chloride, pentafluoropyridine, and 1 M sodium hydroxide solutions were from Wako Pure Chemical Industries (Osaka, Japan). OASIS MAX cartridge (60 mg, 3 mL) was from Waters Corporation (Milford, MA, USA). InertSep SI cartridge (500 mg, 3 mL) was from GL Science (Tokyo, Japan). All other reagents and solvents were of analytical grade.
7α oh dhea
Manufactured by Steraloids
Sourced in United States
7α-OH-DHEA is a chemical compound used in laboratory settings. It is a naturally occurring steroid hormone precursor derived from dehydroepiandrosterone (DHEA). 7α-OH-DHEA serves as a biochemical tool for research and analysis purposes.
Automatically generated - may contain errors
Lab products found in correlation
4 dimethylaminopyridine dmap, by Tokyo Chemical Industry (1 mentions)
Oasis max cartridge, by Waters Corporation (1 mentions)
3β adiol, by Steraloids (1 mentions)
7 oxo dhea, by Steraloids (1 mentions)
D3 dhea, by Steraloids (1 mentions)
D4 cortisol, by CDN Isotopes (1 mentions)
2 hydrazinopyridine, by Merck Group (1 mentions)
16α oh dhea, by Steraloids (1 mentions)
Ammonium formate, by Merck Group (1 mentions)
Trifluoroacetic acid, by Merck Group (1 mentions)
Methyl tert butyl ether (mtbe), by Merck Group (1 mentions)
Diethyl ether, by Merck Group (1 mentions)
Lc ms grade methanol, by Merck Group (1 mentions)
2 protocols using 7α oh dhea
1
Steroid Metabolite Quantification Protocol
2
Steroid Extraction and Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cortisol, cortisone and dehydroepiandrosterone (DHEA) were purchased form Koch-Light Laboratories LTD (Colnbrook, Great Britain), 7α-OH-DHEA, 7β-OH-DHEA, 7-oxo-DHEA, 16α-OH-DHEA and D3-DHEA were from Steraloids (Newport, USA). D4-Cortisol was from CDN isotopes (Ponte-Claire, Canada).
2-hydrazinopyridine, ammonium formate, methyl tertbutyl ether and trifluoroacetic acid were from Sigma-Aldrich (St. Louis, USA). LC-MS grade methanol, water and diethyl ether were from Merck AG (Darmstadt, Germany). The physiological solution (0.9 % sodium chloride) was from B-Braun (Melsungen AG, Germany). [1,2,6,7-3H]Cortisol, specific radioactivity 3.04 TBq/mmol was from Amesrham Biosciences, Inc.
(Amersham, UK).
2-hydrazinopyridine, ammonium formate, methyl tertbutyl ether and trifluoroacetic acid were from Sigma-Aldrich (St. Louis, USA). LC-MS grade methanol, water and diethyl ether were from Merck AG (Darmstadt, Germany). The physiological solution (0.9 % sodium chloride) was from B-Braun (Melsungen AG, Germany). [1,2,6,7-3H]Cortisol, specific radioactivity 3.04 TBq/mmol was from Amesrham Biosciences, Inc.
(Amersham, UK).
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