Different aliquots (0.5–8 ml) of a standard solution containing 40 μg/ml montelukast sodium were moved into sequences of 10 ml volumetric containers, and they were diluted with 0.5% of SLS in water. Determination of montelukast sodium was done by spectrophotometry (Shimadzu UV-1240 model) at 346 nm.[18 ] This experiment was repeated three times a day in 3 consecutive days.
Model uv 1240
Manufactured by Shimadzu
Sourced in Japan
The Shimadzu Model UV-1240 is a single-beam ultraviolet-visible (UV-VIS) spectrophotometer. It is designed to measure the absorbance or transmittance of samples across a range of wavelengths in the ultraviolet and visible light spectrum.
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Lab products found in correlation
6 protocols using model uv 1240
1
Quantifying Montelukast Sodium by UV-Vis
2
Montelukast Sodium ODTs Dissolution
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Montelukast sodium ODTs dissolution test was carried out with United State Pharmacopeia (USP) dissolution apparatus Type II (paddle) at 50 rpm with dissolution medium of SLS 0.5% in purified water at 37°C ± 0.5°C.[26 ] At times of 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, and 12 min, 5 ml sample was removed and substituted by new media. The concentration of samples was measured by ultraviolet (UV) spectrophotometry (Shimadzu UV-1240 model) at 346 nm.
3
Antioxidant Activity of Propolis Extracts
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The seasonality samples (Extracts of Propolis A, Propolis B and Propolis C) were prepared at an initial concentration of 1.0 mg/mL (stock solutions) in the absolute ethanol. An aliquot of 400, 180, 60, 30 and 10 μL was transferred to a volumetric flask of 5 mL, and then 2.0 mL of DPPH solution (3 mM) was added and diluted with absolute ethanol until achieving final concentrations of 80, 36, 12, 6, 3 and 1 μg/mL. The reaction was left to develop in the dark at room temperature (25 °C) over 30 min. The absorbance readings were then performed with a spectrophotometer (Model UV-1240, Shimadzu, Kyoto, Japan) at 518 nm86 (link).
4
Spectrophotometric Quantification of Actinorhodin
5
Antioxidant Activity Evaluation of EEP and NRPE
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Quantitative assessment of the antioxidant activity of EEP and NRPE were performed according to the methods described in the literature [61 (link)] with few modifications. The inhibition of free radical DPPH by the samples was monitored by measuring the decrease in absorbance of solutions with different concentrations. The solvent ethanol was used as blank. A solution of DPPH (3 mM) was prepared transferring 0.0118 g of DPPH reagent to a 100-mL volumetric flask with ethanol.
The EEP and NRPEs were prepared at an initial concentration of 1.0 mg/mL in the solvent system acetone/ethanol (6:4, v/v). An aliquot of 400 μL was transferred to a volumetric flask of 5 mL, and then 2.0 mL of DPPH solution (3 mM) was added and diluted with ethanol until achieving final concentrations of 80.0 μg/mL. The reaction was left to develop in the dark at room temperature (25 °C) over 30 min. The absorbance readings were then performed with a spectrophotometer (Model UV-1240, Shimadzu, Kyoto, Japan) at 518 nm.
The EEP and NRPEs were prepared at an initial concentration of 1.0 mg/mL in the solvent system acetone/ethanol (6:4, v/v). An aliquot of 400 μL was transferred to a volumetric flask of 5 mL, and then 2.0 mL of DPPH solution (3 mM) was added and diluted with ethanol until achieving final concentrations of 80.0 μg/mL. The reaction was left to develop in the dark at room temperature (25 °C) over 30 min. The absorbance readings were then performed with a spectrophotometer (Model UV-1240, Shimadzu, Kyoto, Japan) at 518 nm.
6
Determination of Total Phenolic Content
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The total phenolic content (TPC) was carried out according to Singleton, Orthofer, and Lamuela-Raventos (1999), establishing the gallic acid as the standard [17] . For the determination of the TPC, 0.5 mL of the extracts were used. Then, a volume of 2.5 mL of Folin-Ciocalteau reagent (diluted 1:10 v/v) and 2.0 mL of 4% Na 2 CO 3 (m/v) were mixed. After 2 h of incubation in the dark at room temperature, absorbance was measured on a spectrophotometer (Shimadzu, model UV 1240, Japan) at 740 nm. A blank sample was prepared in the same way but without the sample. The results of TPC were expressed as gallic acid equivalents (mg GAE. g -1 sample), calculated using a curve constructed with concentrations ranging from 2.5 to 40 µg. mL -1 .
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