Multi-channel recordings were made in all subregions of the mPFC in the left hemispheres and/or the contralateral hippocampus passing through the CA1 (Figure 1A ). Single shank 16-channel silicon probes (150 μm inter-site spacing; 25 μm diameter, Atlas Neuroengineering, Leuven, Belgium) were used. Before insertion, the silicon probe was coated with a fluorescent dye (DiI) (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindo-carbocyanine; Molecular probes, Eugene, OR, United States), dissolved in DMSO (1.5–2.5 mg/ml). The signals were passed through a unity-gain headstage (Plexon, TX, United States), and amplified (×1000) and filtered (0.07–300 Hz for LFP) by a Plexon preamplifier (Plexon, TX, United States). The LFP was digitized at 1 kHz, while neuronal spike data were digitized at 40 kHz and recorded using Plexon software (Sort Client).
Unity gain headstage
Manufactured by Plexon
Sourced in United States
The Unity-gain headstage is a compact, low-noise device designed to interface with electrophysiology recording systems. It functions as a signal conditioning unit, providing unity gain amplification to preserve the integrity of the recorded neural signals.
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Lab products found in correlation
3 protocols using unity gain headstage
1
Multi-channel neural recordings in mPFC and hippocampus
2
Electrophysiological Effects of Nicotine in mPFC
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Electrophysiology and surgical methods were similar to that described before (Homayoun and Moghaddam, 2007 (link); Wood et al, 2012 (link)). Electrodes (eight 50 μm wires) were bilaterally implanted in the prelimbic region of the mPFC (+3.0 mm AP, ±0.6 mm ML, −3.2 mm DV) in 15 rats. Recording sessions began after at least five days of recovery. A unity-gain headstage (Plexon, USA) connected to a motorized commutator was used to allow free movement (Figure 1 ). The electrophysiological signal was amplified and filtered for LFP (0.7–300 Hz bandpass) and single-unit activity (150–8000 Hz) via an OmniPlex acquisition system (Plexon). Spikes were sorted using Offline Sorter (Plexon). A recording session included a 30 min baseline, i.p. saline+30 min post-saline, followed by i.p. nicotine+60 min post-nicotine. Locomotor activity was tracked throughout. At the termination of each experiment, animals were anesthetized with chloral hydrate and perfused with saline and 10% formalin. Brains were sectioned and Nissl stained to confirm electrode placements.
3
Extracellular Recordings in Medial Prefrontal Cortex
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Field potential recordings were made from two sites in each subregion of the mPFC: ACC, PrL, IL, and DP in both hemispheres. As each region contained two recording sites these are distinguished throughout as dorsal (d) or ventral (v) for each subregion. Post hoc histology confirmed that the shanks were equidistant from the midline so that the laminar position of the shank was in layer III of the mPFC in both hemispheres. Each of the 16 channels of extracellular signal passed through a unity-gain headstage (Plexon, Dallas, TX) and was then amplified (×1,000) and filtered (0.07–300 Hz for LFP) by a Plexon preamplifier. The LFP was digitized at 1,000 Hz and recorded on a PC (DELL Intel 4-core) running Plexon software (Sort Client).
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