320 a spectrometer

Manufactured by Jasco

Sourced in United States

The Jasco-320-A is a spectrometer designed for precise spectroscopic analysis. It measures the interaction between light and matter, providing quantitative data on the composition and properties of samples. The core function of this instrument is to accurately detect and analyze the absorption, emission, or scattering of light by various substances.

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Lab products found in correlation

Advance 3 600 ascend spectrometer, by Bruker (1 mentions) Jms 600h, by JEOL (1 mentions) Ultra shield plus 500, by Bruker (1 mentions) Uplc rs ultimate 3000 q exactive hybrid quadrupole orbitrap mass spectrometer, by Thermo Fisher Scientific (1 mentions)

5 protocols using 320 a spectrometer

Analytical Characterization of Organic Compounds

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The analytical grade solvents
were used without further purification.
A Bruker spectrometer recorded C¹³ NMR & ¹H NMR spectra at 150 and 600 MHz in deuterated DMSO, respectively.
The Jasco-320A spectrometer recorded IR spectra by the potassium bromide
pellet method. The JMS-HX-110 spectrometer recorded EI-MS spectra.
The TLC plates were viewed in UV light at 254 nm or iodine vapors
using n-hexane and ethyl acetate. Aluminum plates
were pre-coated by silica gel. Melting points were computed through
the Griffin and George apparatus and were uncorrected. All the chemicals
were obtained from Alfa Aesar, Sigma-Aldrich, and Merck.

Abdullah Asif H.M., Kamal S., Rehman A.U., Rasool S, & Hamid Akash M.S. (2022). Synthesis, Characterization, and Enzyme Inhibition Properties of 1,2,4-Triazole Bearing Azinane Analogues. ACS Omega, 7(36), 32360-32368.

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Synthesis and Characterization of Organic Compounds

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Sigma-Aldrich
and Alfa Aesar provided the various chemicals required
to synthesize the targeted molecules. The reaction conditions were
monitored using thin layer chromatography with pre-coated silica gel
G-25-UV254 aluminum plates. Thin layer chromatography was carried
out in a solvent system based on n-hexane and ethyl
acetate, and the results were observed under a UV light with a wavelength
of 254 nm. The KBr pellet method was used to analyze the infrared
(IR) spectra of the compounds in order to identify functional groups
using a JASCO-320 A spectrometer. A Bruker spectrometer running at
500 MHz was used to record ¹H NMR spectra and that at 100
MHz to record ¹³C NMR spectra. The melting points were
recorded using the Griffin and George melting point instrument and
an open capillary tube.

Umair M., Rehman A.U., Abbasi M.A., Siddiqui S.Z., Iqbal J., Khalid H., Rasool S., Khan S.U, & Zafar F. (2023). Modular and Computational Access to Innocuous Multistep Metal-Free Synthesis of 1,3,4-Oxadiazoles as Enzyme Inhibitors. ACS Omega, 8(13), 11952-11965.

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Spectroscopic Characterization of Organic Compounds

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All the chemicals, along
with analytical-grade solvents, were purchased from Sigma Aldrich,
Alfa Aesar (Germany), or Merck through local suppliers. Precoated
silica gel Al plates were used for TLC with ethyl acetate and n-hexane as a solvent system. Spots were detected by UV₂₅₄. A Gallenkamp apparatus was used to detect melting points
in capillary tubes. IR spectra (ν, cm^–1) were
recorded by the KBr pellet method in a Jasco-320-A spectrometer. ¹H NMR spectra (δ, ppm) were recorded at 600 MHz (¹³C NMR spectra, at 150 MHz) in DMSO-d₆ using a Bruker Advance III 600 Ascend spectrometer using
a BBO probe. The electron ionization mass (EI-MS) spectra were measured
on a JEOL JMS-600H instrument with a data processing system.

Abbasi M.A., Rubab K., A.z.i.z.-.u.r.-.R.e.h.m.a.n., Siddiqui S.Z., Hassan M., Raza H., Shah S.A., Shahid M, & Kloczkowski A. (2023). Synthesis and Molecular Docking Studies of Novel Biheterocyclic Propanamides as Antidiabetic Agents Having Mild Cytotoxicity. ACS Omega, 8(25), 22899-22911.

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Characterization of Organic Compounds using Spectroscopic Techniques

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IR spectrum was acquired using a JASCO 320-A spectrometer (JASCO International Co., Ltd., Easton, MD, USA). Normal and reversed-phase silica gels (Merck, Darmstadt, Germany) were used for column chromatography (CC) and thin-layer chromatography (TLC). The compounds were visualized on TLC by spraying with 15% H₂SO₄/ethanol, followed by heating.
NMR spectroscopy was performed using deuterated solvents in an UltraShield Plus 500 (Bruker, Billerica, MA, USA) spectrometer operating at 500 MHz for ¹H and 125 MHz for ¹³C at the College of Pharmacy, Prince Sattam Bin Abdulaziz University. The two-dimensional NMR analyses (COSY, HSQC, and HMBC) were conducted using the standard Bruker pulse program. Chemical shift values are reported in δ (ppm) relative to an internal standard (TMS), and coupling constants (J) are reported in Hertz (Hz).
HRMS was performed using a Thermo Scientific UPLC RS Ultimate 3000 Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Mundelein, IL, USA) combined with high-performance quadrupole precursor selection with high resolution, accurate-mass (HR/AM) Orbitrap™ detection. The instrument was located at Prince Sattam Bin Abdulaziz University, College of Pharmacy. The detection was performed in negative and positive modes, and the experiment run time was 1 min using nitrogen as the supplementary gas with a scan range from 160–1500 m/z.

Al-Massarani S.M., Aldurayhim L.S., Alotaibi I.A., Abdelmageed M.W., Rehman M.T., Basudan O.A., Abdel-Kader M.S., Alajmi M.F., Abdel Bar F.M., Alam P., Al Tamimi M.M, & El Gamal A.A. (2023). Biomarker Quantification, Spectroscopic, and Molecular Docking Studies of the Active Compounds Isolated from the Edible Plant Sisymbrium irio L.. Pharmaceuticals, 16(4), 498.

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Analytical Characterization of Compounds

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Chemicals and solvents of analytical grade were purchased from Sigma Aldrich and Alfa Aesar (Germany).
By using an open capillary tube method, melting points were determined on Griffin and George apparatus and are uncorrected. By using thin layer chromatography (TLC) in various percentages of ethyl acetate and n-hexane as mobile phase, initial purity of compounds was detected at 254 nm. IR peaks were recorded on a Jasco-320-A spectrometer by using a KBr pellet method. 1 H-NMR signals were recorded at 500 MHz in CDCl 3 using Bruker spectrometers. EI-MS signals were recorded by utilizing a JMS-HX-110 spectrometer.

Abbasi A M.u.h.a.m.m.a.d., Hussain G., Rehman A.U., Siddiqui Z S.a.b.a.h.a.t., Shah AA S.y.e.d., Lodhi A M.u.h.a.m.m.a.d., Khan A F.a.r.m.a.n., Ashraf M., Ain Q.U., Ahmad I., Malik R., Shahid M, & Mushtaq Z. (2017). Synthesis of Some Unique Carbamate Derivatives bearing 2-Furoyl-1-piperazine as a Valuable Therapeutic Agents. Acta chimica Slovenica, 64(1).

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