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2 protocols using ammonium perchlorate

1

Endocrine Disruptor Screening Assay

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All reagents were used as received unless otherwise stated. Ammonium perchlorate, bisphenol A (BPA), cycloheximide, diethylstilbestrol (DES), 17β-estradiol (E2), genistein, 4-hydroxytamoxifen (4-OHT), methoxychlor, 4-nonylphenol (4-NP), and sodium azide were purchased from Sigma-Aldrich. ICI 182 780 (ICI) was purchased from Tocris Bioscience; 2,2-bis(4-hydroxyphenyl)-1,1,1-trichloroethane (HPTE) was purchased from Toronto Research Chemicals. Cell culture medium and additives were purchased from Gibco, except for collagen I (Corning), DMSO (Fisher Scientific), and fetal bovine serum (FBS, VWR). CellTiter-Glo, One-Glo, and 5× reporter lysis buffer were purchased from Promega.
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2

Synthesis of Vinyl Pyridine Compound

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2-Vinylpyridine (97%), ammonium perchlorate (99.5%), and perchloric acid (99.99%) were purchased from Sigma-Aldrich Merck. Reagent-grade ultrapure water (nuclease Free) was purchased from Hylabs.
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