Briefly, J774 cells stably expressing Myc-tagged proteins were incubated at 37°C for 10 min in the presence of opsonized Texas Red-conjugated zymosan A (Fujifilm Wako Pure Chemical Industries), as described previously (Sakurai et al., 2018 (link)). The cells were washed in PBS and fixed with 100% methanol for 7 min at −20°C. Subsequently, the cells were incubated with 2% bovine serum albumin (BSA)/PBS for 30 min at 25°C, treated with anti-Myc antibodies, and stained with goat antimouse IgG antibodies conjugated to Alexa 488 (Thermo Fisher Scientific, Waltham, MA). Images were obtained using an LSM780 confocal laser-scanning microscope with a Plan-Apochromat 63 × /1.40 oil DIC M27 objective lens (Carl-Zeiss, Oberkochen, Germany).
Goat antimouse igg antibodies
Manufactured by Thermo Fisher Scientific
Goat anti-mouse IgG antibodies are secondary antibodies that specifically bind to mouse immunoglobulin G (IgG) antibodies. They are used in various immunological techniques, such as Western blotting, ELISA, and immunohistochemistry, to detect and quantify the presence of mouse IgG antibodies in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Ripa buffer, by Thermo Fisher Scientific (2 mentions)
Plan apochromat 63 1.40 oil dic m27 objective lens, by Zeiss (1 mentions)
Alexa 488, by Thermo Fisher Scientific (1 mentions)
Amersham imager 600 system, by GE Healthcare (1 mentions)
Goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
2 protocols using goat antimouse igg antibodies
1
Imaging of Myc-tagged Protein Internalization
2
Protein Lysate Preparation and Western Blot
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Protein lysates of cells were prepared using RIPA buffer (Thermo Fisher Scientific, 89900) and protein concentration was quantified using the BCA protein assay kit (Thermo Fisher Scientific, 23235) after sonication. Primary antibodies diluted in blocking buffer were incubated overnight at 4°C followed by a 2 h incubation of secondary goat-anti-rabbit IgG and goat-anti-mouse IgG antibodies (Thermo Fisher Scientific, 1:10,000) at room temperature. Membranes were visualized using the Amersham Imager 600 system (GE Healthcare, USA).
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