Beriplast p

Female F344/NJcl-rnu/rnu rats at 7 weeks of age (Clea Japan, Tokyo, Japan) were anesthetized by inhalation of isoflurane (1.5%; Mylan Inc., Tokyo, Japan), intubated, and mechanically ventilated. The proximal left anterior descending artery at 2 mm below the left appendage was permanently ligated with a 6–0 polypropylene suture (Ethicon, Johnson & Johnson, USA) under left thoracotomy. Two weeks after infarction, transthoracic echocardiography (ViVid i; GE Healthcare, WI, USA) was performed with an 11.5-MHz transducer, and successfully established heart failure model rats [left ventricle ejection fraction (LVEF) < 50%] were selected. The rats were randomly divided into two groups: the 3D-hiPSC-CT group (n = 10) and the sham surgery control group (n = 10). In the 3D-hiPSC-CT group, the CT was implanted on the surface of the infarct zone of the left ventricle, sutured with 7–0 polypropylene, and covered with pericardium and fibrin glue (Beriplast P: CSL Behring, USA) (Fig 2C). In the sham surgery control group, only fibrin glue was added on the surface of the infarct zone of the heart. At 4 weeks after implantation, the rats were sacrificed under general anesthesia with 5% isoflurane inhalation and the heart was promptly dissected. Another five rats that were implanted with the 3D-hiPSC-CT were observed for 12 weeks and then sacrificed appropriately in the same manner.

Cationic guar gum (Jaguar® Excel; Mw = 1,000,000 − 1,500,000, degree of substitution = 0.10 − 0.13) [15 ] was kindly provided by Sansho Corporation (Tokyo, Japan). Boric acid (H₃BO₃), sodium hydroxide (NaOH), potassium chloride (KCl), sodium phosphate dibasic, potassium phosphate monobasic, sodium chloride (NaCl), Dulbecco’s modified Eagle’s medium (DMEM), penicillin-streptomycin-amphotericin B suspension (PSA-B), and fetal bovine serum (FBS) were purchased from Fujifilm Wako Pure Chemical Industries Ltd. (Osaka, Japan). Fibrin glue, Beriplast® P, was obtained from CSL Behring (Pennsylvania, United States).