Hematoxylin and eosin h e staining solution

The amino-terminated poly (ethylene glycol) (mPEG₄₅-NH₂) was synthesized through the protocol described in our previous work (Chen et al., 2015 (link)). _L-Leucine N-carboxyanhydride (_L-Leu NCA) was obtained from Chengdu Enlai Biological Technology Co., Ltd. (Chengdu, China). Penicillin, streptomycin, trypsin-EDTA (0.05% trypsin and 0.02% EDTA) solution, RPMI 1640 medium, and new-born calf serum (NBCS) were bought from Gibco (Grand Island, NY, United States). Toluene, N,N-dimethylformamide (DMF; anhydrous), diethyl ether, elastase, and chymotrypsin were obtained from Aladdin Reagent Co., Ltd. (Shanghai, China). Hematoxylin and eosin (H&E) staining solution was purchased from Sigma-Aldrich (Shanghai, China). BMS202 was purchased from Selleck Chemicals (United States). PD-L1 antibody and Phospho-VEGF Receptor 2 antibody used for western blot (WB) were purchased from eBioscience (San Diego, CA, United States). PE-cy7-anti-CD45, FITC-anti-CD3, PerCP-cy5.5-anti-CD4, and Pacific Blue-anti-CD8 used for flow cytometry were purchased from eBioscience (San Diego, CA, United States). Enzyme-linked immunosorbent assay (ELISA) kits were purchased from Shanghai Lengton Bioscience Co., Ltd. (Shanghai, China). All the other chemicals were purchased from Beijing Chemical Industry Group Co., Ltd. (China).

Alanine aminotransferases (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), serum γ-glutamyl transpeptidase (GGT), Bilirubin, urea, creatinine and uric acid were from Laboratory Bio-diagnostics Co., Cairo, Egypt. Serum Potassium (K⁺) level was estimated using kits supplied by Spectrum Diagnostics (Egyptian Co. for Biotechnology, Oboor City Industrial Area, Cairo, Egypt), Beta-2 microglobulin (β₂M) using ELISA from Orgentec Diagnostica, Germany. Malondialdhyde (MDA) was from Life Span Biosciences Company (LS.Bio), North America, USA. While, Catalase (CAT), reduced glutathione (GSH) and superoxide dismutase (SOD) were from Shanghai Enzyme-linked Biotechnology Limited Company, Shanghai, China. Ethanol at 100% (St. Louis, MO, USA) was from Algomguria Co, Cairo, Egypt. Egypt’s El Captain Company in Cairo provided Wheat Germ Oil (WGO) (Cap Pharm). Hematoxylin and eosin (H&E) staining solution was obtained by Sigma Aldrich (St. Louis, MO, USA). The Oligod Tprimers, SYBR Green PCR Master Mix, and Qiazol were all supplied by QIAGEN (Valencia, CA, USA). All chemicals used were of molecular grade.