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Dna isolation kit

Manufactured by HiMedia
Sourced in United Kingdom, India

The DNA isolation kit is a laboratory equipment designed to extract and purify DNA from biological samples. It provides a standardized and efficient method for isolating DNA, which is a fundamental step in various molecular biology applications such as genetic analysis, diagnostics, and research.

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2 protocols using dna isolation kit

1

Molecular Characterization of Cowpea Yellow Mosaic Virus

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We surveyed the cowpea crops for yellow mosaic symptoms at the vegetative growth stage in five states of India, namely, Jharkhand, Chhattisgarh, Uttar Pradesh, Madhya Pradesh and Assam, during 2012–13 [43 –44 ]. The maximum incidence of this viral disease was in a field of Jharkhand state, with a disease incidence of about 60–70%. The cowpea plants in the field exhibited stunted growth, yellow patches, mottling of leaves, reduced leaf size and distortion of leaf lamina symptoms (S1 Fig). The infected plant leaf materials were RCA analyzed, cloned and sequenced. Agroinfectious dimeric clones were prepared for MYMIV cowpea isolate, propagated and maintained in cowpea cultivar Pusa Komal through agroinfiltration and maintained in a greenhouse at 25–27°C. Total genomic DNA was extracted from infected leaf samples by using DNA isolation kit (Hi-Media, Mumbai), the purified genomic DNA were subjected to amplify full length of DNA-A and DNA-B, using TempliPhi DNA-Amplification kit (GE Healthcare, UK) through Rolling circle amplification (RCA) method as per manufacturer’s instructions. The ~2.7 Kb DNA fragments obtained after the restriction digestion (EcoRI, HindIII, BamHI, SacI and EcoRV) of the RCA products were cloned and sequenced. The sequences obtained were analyzed using DNA Star, Mega 5.2, and BIOEDIT version 7.0 programs.
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2

DNA Extraction Protocol for Plant Samples

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DNA isolation kit was obtained from Himedia Ltd. Mumbai, India. Schiff's, TTC, NBT, FC reagent, Na 2 CO 3, H 2 O 2 and Galic acid reagents were obtained from Nice Pvt.Ltd. Kerala. Other chemicals used were all of analytical grade.
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