Log In Sign up
The largest database of trusted experimental protocols

Ab226308

Manufactured by Abcam
Visit Supplier

Ab226308 is a recombinant antibody product manufactured by Abcam. It is a laboratory reagent designed for use in research applications.

Automatically generated - may contain errors

Lab products found in correlation

Ab38007, by Cell Signaling Technology (1 mentions) Ab38007, by Abcam (1 mentions) Ab8245, by Abcam (1 mentions)

2 protocols using ab226308

1

Immunohistochemistry Protocol for Quantifying Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
IHC was performed as previously described14 (link). Anti-NFAT5 (1:100, Abcam, ab226308), PGK1 (1:100, Abcam, ab38007), and anti-PCNA (1:500, Cell Signaling Technology, #13110) antibodies were used. Tumor tissues were scored according to the percentage of stained area (0 = 0%, 1 = 1–10%, 2 = 11–50%, 3 = 51–80%, and 4 = 81–100%) and darkness of the nuclei or cytoplasm staining (0 = no staining, 1 = weak staining, 2 = moderate staining, and 3 = strong staining). Final scores were determined by multiplying the 2 numbers mentioned above (“negative” for a score of 0, “weak” for a score of 1–4, “moderate” for a score of 5–8, and “positive” for a score of 9–12). The high-expression group had scores greater than 5, while the low-expression group scores were ≤5.
Jiang Y., He R., Jiang Y., Liu D., Tao L., Yang M., Lin C., Shen Y., Fu X., Yang J., Li J., Huo Y., Hua R., Liu W., Zhang J., Shen B., Zhang Z, & Sun Y. (2019). Transcription factor NFAT5 contributes to the glycolytic phenotype rewiring and pancreatic cancer progression via transcription of PGK1. Cell Death & Disease, 10(12), 948.
+ Open protocol
+ Expand
2

Western Blotting of NFAT5, PGK1

Check if the same lab product or an alternative is used in the 5 most similar protocols
The WB was conducted as reported previously14 (link). Antibodies used were mouse anti-GAPDH (1:10,000, Abcam, ab8245), rabbit anti-NFAT5 (1:1000, Abcam, ab226308), and rabbit anti-PGK1 (1:500, Abcam, ab38007).
Jiang Y., He R., Jiang Y., Liu D., Tao L., Yang M., Lin C., Shen Y., Fu X., Yang J., Li J., Huo Y., Hua R., Liu W., Zhang J., Shen B., Zhang Z, & Sun Y. (2019). Transcription factor NFAT5 contributes to the glycolytic phenotype rewiring and pancreatic cancer progression via transcription of PGK1. Cell Death & Disease, 10(12), 948.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!