Log In Sign up
The largest database of trusted experimental protocols

Pten floxed mice

Manufactured by Jackson ImmunoResearch
Visit Supplier
Sourced in Montenegro

Pten-floxed mice are a type of genetically modified mouse model. They have the Pten gene flanked by loxP sites, which allows for conditional deletion of the Pten gene in target tissues or cell types when crossed with Cre recombinase expressing mice.

Automatically generated - may contain errors

Lab products found in correlation

D12492, by Research Diets (2 mentions) Tre cre, by Jackson ImmunoResearch (2 mentions) B6 sjl mice, by Jackson ImmunoResearch (1 mentions) Cd4 cre transgenic mice, by Jackson ImmunoResearch (1 mentions) Ot 1 tcr transgenic mice, by Jackson ImmunoResearch (1 mentions) Rag1 ko mice, by Jackson ImmunoResearch (1 mentions) Ot 2 tcr transgenic mice, by Jackson ImmunoResearch (1 mentions)

4 protocols using pten floxed mice

1

Senp7-floxed Mice and T Cell Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Senp7-floxed mice were generated at the GemPharmatech using a LoxP-targeting system. The Senp7-floxed mice were crossed with Cd4-Cre transgenic mice (The Jackson Laboratory) to produce age-matched Senp7fl/fl and Senp7fl/flCd4-Cre mice. The Pten-floxed mice (The Jackson Laboratory) were crossed with Senp7fl/flCd4-Cre mice to produce Senp7+/+Ptenfl/flCd4-Cre (Pten-KO) and Senp7fl/flPtenfl/flCd4-Cre (DKO) mice. Rag1-KO mice, OT-I TCR–transgenic mice, OT-II TCR–transgenic mice, and B6.SJL mice were from The Jackson Laboratory. Male and female mice were sex matched and used at 6 to 10 weeks of age. Mice were maintained in a specific pathogen–free (SPF) facility at the Shanghai Jiao Tong University School of Medicine.
Wu Z., Huang H., Han Q., Hu Z., Teng X.L., Ding R., Ye Y., Yu X., Zhao R., Wang Z, & Zou Q. (2022). SENP7 senses oxidative stress to sustain metabolic fitness and antitumor functions of CD8+ T cells. The Journal of Clinical Investigation, 132(7), e155224.
+ Open protocol
+ Expand
2

Conditional Knockout Mouse Models

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The Klf5 floxed mouse strain, in which the genomic DNA from exon 2 to exon 3 of Klf5 is flanked by loxP sites, was generated as described in our previous study [25] (link). The PB-Cre4 transgenic mouse strain, where expression of Cre recombinase is driven by the prostatic epithelial specific probasin promoter [30] (link), was purchased from the NCI Mouse Models of Human Cancers Consortium (MMHCC, Frederick, MD; Cat. No. 01XF5). Pten floxed mice were purchased from the Jackson Laboratory (Bar Harbor, ME; Cat. No. 004597). All mice were maintained in a mixed background of C57BL/6 J and 129Sv/J.
Xing C., Ci X., Sun X., Fu X., Zhang Z., Dong E.N., Hao Z.Z, & Dong J.T. (2014). Klf5 Deletion Promotes Pten Deletion–Initiated Luminal-Type Mouse Prostate Tumors through Multiple Oncogenic Signaling Pathways. Neoplasia (New York, N.Y.), 16(11), 883-899.
+ Open protocol
+ Expand
3

Inducible Adiponectin Knockout Mouse Model

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Animal care and experimental protocols were approved by the Institutional animal care and use committee of the University of Texas Southwestern medical center. Adiponectin-RTTA mice were produced as previously described (9 (link)). Tre-Cre (Stock Number: 006234) and PTEN floxed mice (Stock Number: 006440) were purchased from Jackson laboratories. All experiments were done using male mice with littermate controls that were adiponectinP-rtTA or Tre-Cre negative. Mice were fed a standard chow diet (number 5058, LabDiet), a Dox-chow diet (600 mg kg−1 Dox; Bio-Serv), a 60% HFD (D12492, Research Diets Inc.) or a Dox-HFD (600 mg kg−1 Dox; Bio-Serv). Following weaning mice were randomly placed into cages for the remainder of their life before genotyping was performed. No other randomization was used while conducting experiments. No animals were excluded from any experiments unless they displayed obvious wounds from fighting as determined by our veterinarians.
Morley T.S., Xia J.Y, & Scherer P.E. (2015). Selective enhancement of insulin sensitivity in the mature adipocyte is sufficient for systemic metabolic improvements. Nature Communications, 6, 7906.
+ Open protocol
+ Expand
4

Adiponectin Regulation of PTEN in Mice

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Animal care and experimental protocols were approved by the Institutional Animal Care and Use Committee of the University of Texas Southwestern Medical Center. Adiponectin-rtTA mice were produced as previously described10 (link). Tre-Cre (Stock number: 006234) and PTEN-floxed mice (Stock number: 006440) were purchased from Jackson laboratories. All experiments were done using male mice with littermate controls that were adiponectinP-rtTA or Tre-Cre negative. Mice were fed a standard chow diet (number 5058, LabDiet), a Dox-chow diet (600 mg kg−1 Dox; Bio-Serv), a 60% HFD (D12492, Research Diets Inc.) or a Dox-HFD (600 mg kg−1 Dox; Bio-Serv). Following weaning, mice were randomly placed into cages for the remainder of their life before genotyping was performed. No other randomization was used while conducting experiments. No animals were excluded from any experiments, unless they displayed obvious wounds from fighting as determined by our veterinarians.
Morley T.S., Xia J.Y, & Scherer P.E. (2015). Selective enhancement of insulin sensitivity in the mature adipocyte is sufficient for systemic metabolic improvements. Nature Communications, 6, 7906.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!