Bovine brain l α phosphatidyl l serine

Egg yolk L-α-phosphatidylcholine (PC), cardiolipin (CL) from E.coli, bovine brain L-α-phosphatidyl-L-serine (PS), egg yolk L-α-phosphatidic acid (PA), and potassium ferricyanide were purchased from Sigma Chemical Co. (St. Louis, MO). All phospholipids were further purified on silica columns. Central Asian cobra Naja naja oxiana crude venom was obtained as a gift from Prof. L.Ya. Yukelson (Institute of Biochemistry, Uzbekistan Academy of Sciences) in lyophilized form. Cardiotoxins CTII and CTI, also known as cytotoxin CTII (or Vc5) and cytotoxin CTI (or Vc1) respectively, were isolated from 500 mg of crude venom as previously published [23 (link)] and were further purified by cation exchange HPLC by using a SCX 83-C-13-ET1 Hydropore column as previously described [24 (link)]. The purity of CTII and its molecular weight were assessed by Native-PAGE (Fig. 5C). The molecular weight of CTII is approximately 7 kDa. Based on the Native-PAGE assay, the purity of “gradient-purified” CTII is high (~99%) based on the absence of noticeable contaminating protein bands (Fig. 5C, lane B). All other reagents were from Sigma-Aldridge (Moscow, Russia).