First, 1 mg of each dried potato sample was carefully weighed and dissolved in 1 mL MeOH acidified with 0.1% formic acid. The 0.1 mg/mL samples were obtained by 1:10 dilution of the prepared stock solution and were submitted to LC-MS analysis. All samples were analyzed in triplicate. Identification of compounds was confirmed by comparison with standards.; 3-O-caffeoylquinic acid (1 ), 5-O-caffeoylquinic acid (2 ), rutin (6 ), quercetin-3-O-glucoside (7 ), cyanidin-3-O-glucoside and quercetin-3-O-sulfate (8 ) were purchased from Extrasynthese (Lyon, France). Feruloyl quinic acid (4 ), and quercetin 3-O-(2′-glucosyl)-rutinoside (5 ) were isolated in our lab in previous studies.
3 o caffeoylquinic acid
Manufactured by Extrasynthese
Sourced in France
3-O-caffeoylquinic acid is a compound that can be used as a laboratory standard or reference material. It is a naturally occurring phenolic compound found in various plant species.
Automatically generated - may contain errors
Lab products found in correlation
Quercetin 3 o glucoside, by Extrasynthese (4 mentions)
5 o caffeoylquinic acid, by Extrasynthese (3 mentions)
Cyanidin 3 o glucoside, by Extrasynthese (3 mentions)
Ferulic acid, by Extrasynthese (3 mentions)
P coumaric acid, by Extrasynthese (3 mentions)
Caffeic acid, by Extrasynthese (2 mentions)
Methanol, by Merck Group (2 mentions)
Formic acid, by Merck Group (2 mentions)
Rutin, by Extrasynthese (1 mentions)
Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions)
Magnesium chloride hexahydrate, by Avantor (1 mentions)
Kaempferol 3 o glucoside, by Extrasynthese (1 mentions)
Apigenin 8 c glucoside, by Extrasynthese (1 mentions)
Apigenin 6 c glucoside, by Extrasynthese (1 mentions)
Kaempferol 3 o rutinoside, by Extrasynthese (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
Formic acid, by Carlo Erba (1 mentions)
Sodium carbonate, by Merck Group (1 mentions)
Suprapur nitric acid, by Merck Group (1 mentions)
3 4 di o caffeoylquinic acid, by Merck Group (1 mentions)
7 protocols using 3 o caffeoylquinic acid
1
Potato Phenolic Compound Analysis
2
Antioxidant and Cholinesterase Inhibition Assays
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Reagents for antioxidant activities and cholinesterase inhibition assays were from Sigma-Aldrich (St. Louis, MO, USA and Steinheim, Germany), except magnesium chloride hexahydrate, which was bought from VWR (Leuven, Belgium), sodium chloride from Fisher Scientific (Fair Lawn, NJ, USA), sodium nitroprusside dihydrate from Fluka, and sodium carbonate from Merck. Hydrogen peroxide 35.5% w/w was purchased from Labchem (Zelienople, PA, USA). Methanol Chromasolv for HPLC was from Riedel-de Haën (Seelze, Germany), and formic acid was from Carlo Erba (Val de Reuil, France). The standards used for HPLC analyses were acquired from Sigma-Aldrich (4-O-caffeoylquinic acid, quercetin-3-O-rutinoside hydrate, protocatechuic acid, ferulic acid, and p-coumaric acid), Fluka (caffeic acid), Alfa Aesar (5-O-caffeoylquinic acid), and Extrasynthèse (Genay, France): 3-O-caffeoylquinic acid, apigenin-8-C-glucoside, apigenin-6-C-glucoside, quercetin-3-O-glucoside, kaempferol-3-O-glucoside, kaempferol-3-O-rutinoside, and tiliroside. Ultrapure water (with resistivities of 18.2 MΩ/cm) was obtained using a Milli-Q water purification system from Millipore (Molsheim, France). Standard solutions of Pb and Cd, Atomic Absorption standard with 4% HNO3, were purchased from SPC Science (Quebec, Canada). Suprapur nitric acid 65% (v/v) and NH4H2PO4 (Suprapur) were from Merck (Darmstad, Germany).
3
Quantification of Caffeic Acid Derivatives
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Standards of caffeic acid, 4-O-caffeoylquinic acid, 5-O-caffeoylquinic acid, 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid, and 2,4,5-trimethoxycinnamic acid were purchased from Sigma Aldrich Co. (St. Louis, MO, USA); 1-O-caffeoylquinic acid, 3-O-feruloylquinic acid, 4-O-feruloylquinic acid, 5-O-feruloylquinic acid, 3-O-caffeoylquinic acid methyl ester, 3,4-di-O-caffeoylquinic acid methyl ester, 3,5-di-O-caffeoylquinic acid methyl ester, and 4,5-di-O-caffeoylquinic acid methyl ester were purchased from Chem Face (Wuhan, China); p-coumaric acid, ferulic acid, 3-O-caffeoylquinic acid and 5-O-caffeoylquinic acid methyl ester, and 1,3-di-O-caffeoylquinic acid from Extrasynthese (Genay Cedex, France); and 1,5-di-O-caffeoylquinic acid were purchased from Avention (Incheon, Republic of Korea).
4
Phenolic Compounds and Metabolites Quantification
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Chemicals and materials for cell culture were purchased from VWR International (Milan, Italy). The MTS cell proliferation assay kit was obtained from Promega (Milan, Italy). Mass spectrometry solvents were purchased from Bio-Rad (Hercules, CA, USA). The parent phenolic compounds 3-O-caffeoylquinic acid (purity ≥ 99%), 5-O-caffeoylquinic acid (purity ≥ 99%), 3,5-O-dicaffeoylquinic acid (purity ≥ 97%), quercetin (purity ≥ 99%), quercetin-4′-O-glucoside (purity ≥ 99%) and quercetin-3-O-glucoside-4′-O-glucoside (purity ≥ 98.5%) were obtained from Extrasynthese (Genay, France). Phenolic compound metabolites 3-(3′-hydroxyphenyl)propanoic acid (purity ≥ 98%), 3-(3′-hydroxyphenyl)acetic acid (purity ≥ 99%) and 3-(3′,4′-dihydroxyphenyl)acetic acid (purity ≥ 98%) were purchased from Thermo-Fisher Scientific (Waltham, MA, USA). All the other chemicals were purchased from Sigma-Aldrich (Milan, Italy).
5
Analytical Quantification of Phenolic Compounds
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Ethanol absolute was purchased from VWR (Milano, Italy); methanol and 85% w/w phosphoric acid were purchased from Sigma-Aldrich (Steinheim, Germany). LC-MS grade acetonitrile, formic acid, and water were purchased from Merck (Darmstadt, Germany). Standards of cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside, peonidin-3-O-glucoside, peonidin-3-O-rutinoside, quercetin-3-O-rutinoside, quercetin-3-O-glucoside, quercetin-3-O-rhamnoside, p-coumaric acid, ferulic acid, vanillic acid, 3-O-caffeoylquinic acid (neochlorogenic acid), and 5-O-caffeoylquinic acid (chlorogenic acid) were purchased from Extrasynthese (Genay, France) and TransMIT (Giessen, Germany).
Soy lecithin (a mixture of polar phospholipids and glycolipids) and propylene glycol (PG) were purchased from Galeno Srl (Comeana, Prato, Italy).
Soy lecithin (a mixture of polar phospholipids and glycolipids) and propylene glycol (PG) were purchased from Galeno Srl (Comeana, Prato, Italy).
6
Phytochemical and Antioxidant Assays
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We purchased 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), acetone, acetonitrile, citric acid, DPPH, ferrous-ion-o-dianisidine, Folin–Ciocalteu phenol reagent, formic acid, gallic acid, hydrochloric acid, methanol, β-nicotinamide adenine dinucleotide 2-phosphate reduced tetrasodium salt (NADPH), ultrapure phenolic standards, 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole (Purpald), sodium acetate, superoxide dismutase determination kit, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), and 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Ascorbic acid, boric acid, ferric chloride, ferrous sulfate, hydrogen peroxide, potassium chloride, sodium carbonate, sodium hydroxide, and sulfuric acid were obtained from Vetec (Rio de Janeiro, RJ, Brazil). Cyanidin-3-O-glucoside, delphinidin-3-O-glucoside, delphinidin, quercetin-3-O-glucoside, 3-O-caffeoyl-quinic acid, and myricetin were purchased from Extrasynthese (Genay, France). HA, PCA, VA, isovanillic acid (iVA), ellagic acid, FA, and kaempferolwere purchased from Sigma-Aldrich (St Louis, MO, USA). The kit to analyze urine creatinine was purchased from Labtest Diagnóstica S.A. (Lagoa Santa, MG, Brazil).
7
Analytical Protocols for Phenolic Compounds
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Acetonitrile 99.9% of high-performance liquid chromatography (HPLC) grade was purchased from Fisher Scientific (Lisbon, Portugal). Phenolic compound standards (caffeic acid, ferulic acid, 3-O-caffeoylquinic acid, p-coumaric acid and rosmarinic acid) were acquired from Extrasynthese (Genay, France). Water was treated in Milli-Q water purification system (TGI Pure Water Systems, Greenville, SC, USA). Ferrous ammonium sulfate(II) hexahydrate, sodium chloride and sulfuric acid, all with PA purity, were purchased from Panreac S.A. (Barcelona, Spain) (proanalysis).
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