FBS, penicillin, and streptomycin were purchased from HyClone Laboratories. RPMI 1640 medium and charcoal-stripped FBS were purchased from Thermo Fisher. Dialyzed FBS was from Life Technologies. Optima ammonium acetate, ammonium hydroxide, Optima liquid chromatography–mass spectrometry (LC-MS) grade, acetonitrile, methanol, and water were purchased from Fisher Scientific. U-13C glucose, U-13C glutamine, and 2,3,3-2H serine were obtained from Cambridge Isotope Laboratories. U-13C palmitate, C16-ceramide, and C2-ceramide were purchased from Sigma-Aldrich.
Optima ammonium acetate
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Optima ammonium acetate is a high-purity laboratory reagent used as a buffer in various analytical and biochemical applications. It is a salt comprised of ammonium and acetate ions, designed to maintain a specific pH range in solutions.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (4 mentions)
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
Ammonium hydroxide, by Thermo Fisher Scientific (2 mentions)
U 13c glucose, by Cambridge Isotopes (2 mentions)
C2 ceramide, by Merck Group (2 mentions)
U 13c glutamine, by Cambridge Isotopes (2 mentions)
Dialyzed fbs, by Thermo Fisher Scientific (2 mentions)
Optima liquid chromatography mass spectrometry lc ms grade, by Thermo Fisher Scientific (2 mentions)
2 3 3 2h serine, by Cambridge Isotopes (2 mentions)
C16 ceramide, by Merck Group (2 mentions)
U 13c palmitate, by Merck Group (2 mentions)
Charcoal stripped fbs, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Cytiva (2 mentions)
Penicillin, by Cytiva (2 mentions)
Streptomycin, by Cytiva (2 mentions)
Milli q water purification system, by Merck Group (1 mentions)
3 protocols using optima ammonium acetate
1
Metabolomic Analysis of Cell Lines
2
Metabolomic Analysis of Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
FBS, penicillin, and streptomycin were purchased from HyClone Laboratories. RPMI 1640 medium and charcoal-stripped FBS were purchased from Thermo Fisher. Dialyzed FBS was from Life Technologies. Optima ammonium acetate, ammonium hydroxide, Optima liquid chromatography–mass spectrometry (LC-MS) grade, acetonitrile, methanol, and water were purchased from Fisher Scientific. U-13C glucose, U-13C glutamine, and 2,3,3-2H serine were obtained from Cambridge Isotope Laboratories. U-13C palmitate, C16-ceramide, and C2-ceramide were purchased from Sigma-Aldrich.
3
HPLC-MS Compound Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
High performance liquid chromatography (HPLC) grade methanol, acetonitrile, and Liquid chromatography-mass spectrometry (LC-MS) grade (Optima™) ammonium acetate were purchased from Fisher Scientific (Loughborough, UK). A total of 141 compounds were targeted in this study. All analytical standards were of a purity of ≥97%. Ultra-pure water was obtained from a Millipore Milli-Q water purification system with a specific resistance of 18.2 MΩ cm or greater (Millipore, Bedford, MA, USA). Stock solutions (1 mg mL−1) were prepared in methanol (MeOH) or acetonitrile (MeCN) and stored in silanised amber vials (20 mL). Working solutions were prepared daily in ultra-pure water, as required. All solutions were stored at −20 °C and in the dark to reduce possible degradation.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!