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Alexafluor 647 conjuaged goat anti rabbit antibody

Manufactured by Thermo Fisher Scientific
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The AlexaFluor-647-conjugated goat anti-rabbit antibody is a secondary antibody used for immunodetection and immunolocalization applications. It is designed to bind to and detect rabbit primary antibodies, allowing for the visualization of target proteins or other biomolecules in various experimental techniques.

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Lab products found in correlation

Methylbutane, by Merck Group (2 mentions) Rat anti mouse cd16 cd32, by BD (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Polyclonal guinea pig anti insulin antibody, by Agilent Technologies (2 mentions) Alexafluor 555 conjugated anti guinea pig antibody, by Thermo Fisher Scientific (2 mentions) Tcs sp5 confocal microscopy, by Leica camera (2 mentions)

2 protocols using alexafluor 647 conjuaged goat anti rabbit antibody

1

Islet Cryopreservation and Immunofluorescence Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
After the observation period of 200 days, long-term islet bearing kidneys were snap frozen in OCT compound (Sakura Tissue-Tek) by submerging in methyl butane (Sigma) on dry ice. Tissues were cut in 10 µm-thick slices using a Bright OTF5000 cryomicrotome (Rose Scientific) and put on frosted slides for staining. Slides were fixed in 4% paraformaldehyde, incubated in 0.5% Triton X-100, and blocked in 0.1% bovine serum albumin, 5% goat serum, and rat anti-mouse CD16/CD32 (BD Pharmingen). Staining was performed using rabbit anti-glucagon monoclonal antibody (Cell Signaling 8233S, 1:100) and guinea pig anti-insulin polyclonal antibody (Dako A0564, 1:100) as primary antibodies, followed by washing and staining with AlexaFluor-647-conjuaged goat anti-rabbit antibody (Life Technologies A21244, 1:100) and AlexaFluor-555-conjugated anti-guinea pig antibody (Invitrogen A21435, 1:300). Hoechst 33342 (Molecular Probes H3570, 1:25) was used to stain DNA. Fluorescent images were obtained using a Leica TCS SP5 confocal microscopy under 10X magnification.
Headen D.M., Woodward K.B., Coronel M.M., Shrestha P., Weaver J.D., Zhao H., Tan M., Hunckler M.D., Bowen W.S., Johnson C.T., Shea L., Yolcu E.S., García A.J, & Shirwan H. (2018). Local immunomodulation with Fas ligand-engineered biomaterials achieves allogeneic islet graft acceptance. Nature materials, 17(8), 732-739.
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2

Islet Cryopreservation and Immunofluorescence Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
After the observation period of 200 days, long-term islet bearing kidneys were snap frozen in OCT compound (Sakura Tissue-Tek) by submerging in methyl butane (Sigma) on dry ice. Tissues were cut in 10 µm-thick slices using a Bright OTF5000 cryomicrotome (Rose Scientific) and put on frosted slides for staining. Slides were fixed in 4% paraformaldehyde, incubated in 0.5% Triton X-100, and blocked in 0.1% bovine serum albumin, 5% goat serum, and rat anti-mouse CD16/CD32 (BD Pharmingen). Staining was performed using rabbit anti-glucagon monoclonal antibody (Cell Signaling 8233S, 1:100) and guinea pig anti-insulin polyclonal antibody (Dako A0564, 1:100) as primary antibodies, followed by washing and staining with AlexaFluor-647-conjuaged goat anti-rabbit antibody (Life Technologies A21244, 1:100) and AlexaFluor-555-conjugated anti-guinea pig antibody (Invitrogen A21435, 1:300). Hoechst 33342 (Molecular Probes H3570, 1:25) was used to stain DNA. Fluorescent images were obtained using a Leica TCS SP5 confocal microscopy under 10X magnification.
Headen D.M., Woodward K.B., Coronel M.M., Shrestha P., Weaver J.D., Zhao H., Tan M., Hunckler M.D., Bowen W.S., Johnson C.T., Shea L., Yolcu E.S., García A.J, & Shirwan H. (2018). Local immunomodulation with Fas ligand-engineered biomaterials achieves allogeneic islet graft acceptance. Nature materials, 17(8), 732-739.
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