Qp5000 plus

CZ-EO was dissolved in ethyl ether and analyzed by gas chromatography flame- ionization detection (GC-FID) and gas chromatography-mass spectrometry (GC–MS) on the Shimadzu QP5000 Plus and GCMS2010 Plus (Shimadzu Corporation, Kyoto, Japan) systems, respectively. During GC-FID, the column temperature was programmed to rise from 60 to 240 °C at 3 °C/min and held at 240 °C for 5 min. H₂ was used as the carrier gas at a flow rate of 1.0 mL/min. The equipment was set to operate in the injection mode; the injection volume was 0.1 µL (split ratio of 1:10); and the injector and detector temperatures were 240 and 280 °C, respectively. Relative concentrations of the CZ-EO compounds were estimated from the relative peak areas (%) of the GC-FID chromatograms, and expressed as the average of triplicate analyses and the corresponding standard deviations. GC-MS conditions and the identification were based on a previously reported methodology [17 (link)]. The volatile components in CZ-EO (Table 1) were identified on the basis of their retention indices on an Rtx-5MS (30 m × 0.25 mm; 0.250 µm) capillary column, under the same operating conditions used for the GC relative to a homologous series of n-alkanes (C₈–C₂₀). The structures were computer matched with Wiley 7, NIST 08, and FFNSC 1.2, and their fragmentation patterns were compared with literature data [21 ].