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Cd4 fitc

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CD4-FITC is a fluorescent-labeled antibody that binds to the CD4 surface protein expressed on certain T cells. It can be used for the identification and enumeration of CD4+ T cells in flow cytometry applications.

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Lab products found in correlation

Cd3 fitc, by BD (1 mentions) Cd3 efluor 450, by BD (1 mentions) Foxp3 apc, by Miltenyi Biotec (1 mentions) Foxp3 pe, by Miltenyi Biotec (1 mentions) Cd45ra pe, by Miltenyi Biotec (1 mentions) Cd45ra apc, by Miltenyi Biotec (1 mentions) Cd62l apc, by Miltenyi Biotec (1 mentions) Cd4 percp cyanine5, by BD (1 mentions) Cd8 pe cyanine7, by BD (1 mentions) Cd45 apc, by R&D Systems (1 mentions) Cd14 pe, by R&D Systems (1 mentions) Cd25 pe, by Miltenyi Biotec (1 mentions) Alexa fluor 568, by Thermo Fisher Scientific (1 mentions) Cd19 apc, by Thermo Fisher Scientific (1 mentions) Alexa fluor 633 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Cd14 fitc, by BD (1 mentions) Cd19 fitc, by BD (1 mentions) Ccr7 apc cy7, by BioLegend (1 mentions) Igd fitc, by BD (1 mentions) Cd45 percp cy5, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

CD4+/FITC staining reagent (2 citations) Anti-human CD4-FITC (1 citations)

3 protocols using cd4 fitc

1

Multicolor Flow Cytometry Analysis of Immune Cells

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Cells were stained with CD3 FITC (Cat# 11-0039), CD3 eFluor 450 (Cat# 48-0038), CD4 PE-Cyanine7 (Cat# 25-0049), CD4 PerCP-Cyanine5.5 (Cat# 560650, BD Pharmingen), Foxp3 APC (Cat# 17-4777), Foxp3 PE (Cat# 12-4776), CD45RA PE (Cat# 130-092-248, Miltenyi), CD45RA APC (Cat# 130-092-249, Miltenyi), CD62L APC (Cat# 17-0629), CD25 PE (Cat# 130-091-024, Miltenyi), CD25 APC-H7 (Cat# 560225, BD Pharmingen), CD8 PE-Cyanine7 (Cat# 25-0088), Perforin PE (Cat# 12-9994), granzyme B PE (Cat# 12-8899), CCR8 APC (Cat# FAB1429A, R&D System), CD4 FITC (Cat# 11-0049), CD45 APC (Cat# 17-9459), CD14 PE (Cat# 12-0149), CD45RO PE (Cat# 12-0457), CD45RO eFluor 450 (Cat# 48-0457), CD127 APC (Cat# 17-1278). PITPNM3 antibody (Cat# NBP1-31070, Novus) was labeled with APC by Abcam APC Conjugation Kit (ab201807, Abcam) according to the manufacturer's instructions. For the intracellular stain, cells were pretreated with Intracellular Fixation and Permeabilization kit (Cat# 88-8824) according to the manufacturer's instructions. All the reagents were from eBioscience unless indicated otherwise. Cells were subsequently analyzed by multicolor flow cytometry (Gallios, Beckman Coulter, China).
Su S., Liao J., Liu J., Huang D., He C., Chen F., Yang L., Wu W., Chen J., Lin L., Zeng Y., Ouyang N., Cui X., Yao H., Su F., Huang J.D., Lieberman J., Liu Q, & Song E. (2017). Blocking the recruitment of naive CD4+ T cells reverses immunosuppression in breast cancer. Cell Research, 27(4), 461-482.
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2

Multiparametric Flow Cytometry for Cell Surface and Intracellular Markers

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Anti-ICOSL (MIH12), ICOSL-APC, CD16-PE, anti–VCAM-1-PE, anti–E-Selectin-PE, CD27-PE, CD19-APC, CD45-PerCP/Cy5.5, goat anti-rabbit Alexa Fluor 488, goat anti-mouse Alexa Fluor 633, and Alexa Fluor 568 were from Thermo Fisher Scientific. CD19-FITC, IgD-FITC, FoxP3-A647, CD14-FITC, and CD45RA-PE were from BD Biosciences. Antibodies against RCAS1 and β-actin were from Cell Signaling Technology. HLA-DR-PerCP, CD11c-Pacific Blue, CXCR5-BV421, CCR7-APC/Cy7, PD1-PE/Cy7, and ICOS-APC were from BioLegend. BDCA1 (CD1c)-APC, BDCA3 (CD141)-PE, CD4-FITC, and ICAM-1-FITC were from R&D Systems.
Roussel L., Landekic M., Golizeh M., Gavino C., Zhong M.C., Chen J., Faubert D., Blanchet-Cohen A., Dansereau L., Parent M.A., Marin S., Luo J., Le C., Ford B.R., Langelier M., King I.L., Divangahi M., Foulkes W.D., Veillette A, & Vinh D.C. (2018). Loss of human ICOSL results in combined immunodeficiency. The Journal of Experimental Medicine, 215(12), 3151-3164.
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3

IL-23R Expression in CD4+ Cells

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PBMCs were isolated from HCs (n = 8) and SLT patients (n = 7), and stained for CD4 (CD4-FITC) and the IL-23 receptor (IL23R-PE; R&D Systems, cat#FAB14001P) and analysed with FACS. The percentage of CD4+ and CD4− cells expressing the IL23-R were determined and compared between groups.
Evans R.D., Antonelou M., Sathiananthamoorthy S., Rega M., Henderson S., Ceron-Gutierrez L., Barcenas-Morales G., Müller C.A., Doffinger R., Walsh S.B, & Salama A.D. (2020). Inherited salt-losing tubulopathies are associated with immunodeficiency due to impaired IL-17 responses. Nature Communications, 11, 4368.
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