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Prl tk renilla luciferase prl tk luc

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The pRL-TK Luc is a plasmid containing the Renilla luciferase gene under the control of the herpes simplex virus thymidine kinase (HSV-TK) promoter. The Renilla luciferase is a bioluminescent reporter enzyme that can be used to quantify gene expression.

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Lab products found in correlation

Pcdna3, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

PRL-TK-Luc Renilla luciferase PRL-TK Renilla luciferase PRL-TK Renilla PRL-TK-luc Renilla-luciferase (pRL) PRL-TK Renilla-TK TK-luc Renilla luciferase

2 protocols using prl tk renilla luciferase prl tk luc

1

Plasmid Transfection Efficiency Optimization

Cited 1 time
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The expression vector pcDNA3 was purchased from Invitrogen. An internal control plasmid for transfection efficiency, pRL-TK renilla luciferase (pRL-TK Luc), was purchased from Promega. The synthetic vitellogenin 3xERE-TATA fused to a luciferase reporter gene plasmid, 3xERE Luc, and a full-length human ERα expression plasmid, pcDNA/WT ERα, and pcDNA/SRC-2 expression plasmids have been described previously (51 (link)). All the Q375 mutant expression plasmids used were generated by Celplor Inc. (www.celplor.com) using the pENTR system.
Li Y., Coons L.A., Houtman R., Carlson K.E., Martin T.A., Mayne C.G., Melchers D., Jefferson T.B., Ramsey J.T., Katzenellenbogen J.A, & Korach K.S. (2020). A mutant form of ERα associated with estrogen insensitivity affects the coupling between ligand binding and coactivator recruitment. Science signaling, 13(650), eaaw4653.
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2

Plasmid Transfection Efficiency Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
The expression vectors pcDNA3 was purchased from Invitrogen. An internal control plasmid for transfection efficiency, pRL-TK renilla luciferase (pRL-TK Luc), was purchased from Promega. The luciferase reporter plasmid, 3xERE Luc (synthetic vitellogenin ERE-TATA box sequence fused to a luciferase reporter gene), pcDNA/hERα , and pcDNA/hERβ (full-length human ER expression plasmids) have been described previously. The following plasmids were gifts from D. McDonnell (Duke University): pcDNA/SRC-1, -2, -3, and pcDNA/PGC1α , β .
Li Y., Perera L., Coons L.A., Burns K.A., Tyler Ramsey J., Pelch K.E., Houtman R., van Beuningen R., Teng C.T, & Korach K.S. (2018). Differential in Vitro Biological Action, Coregulator Interactions, and Molecular Dynamic Analysis of Bisphenol A (BPA), BPAF, and BPS Ligand–ER Complexes. Environmental Health Perspectives, 126(1), 017012.
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