Daunorubicin

Cell proliferation was determined with the Cell Counting Kit-8 (CCK8, Dojin Laboratories, Kumamoto, Japan) assay. Briefly, 4 × 10E+4 cells were seeded into each well of 96-well plates. 2, 4 or 7 d later 10 μl of the kit reagent was added to each well and 2 h later all plates were scanned by a microplate reader at 450 nm. CCK8 was also used to determine cell viability after drug exposures including daunorubicin, dexamethasone, methotrexate, cytarabine and imatinib (Solarbio, Beijing, China). Cells were seeded and 72 h later 10 μl of the kit reagent was added to each well and 2 h later plates were scanned by a microplate reader at 450 nm. Cell viability was assessed based on the value of fluorescent signal of live cells with no drug treatment. Experiments were performed in triplicate for 3 times independently.

Cell viability was measured by CCK-8 Assay Kit (Beyotime, Shanghai, China). Cells were seeded in 96-well plates at a density of 5 × 10³ cells/well. Twelve hours later, the final concentrations of F1–F7 were adjusted to 6.25, 12.5, 25, 50, 100 µM, and Daunorubicin (Solarbio, Beijing, China) was adjusted to 0.0625, 0.125, 0.25, 0.5, 1 µM, and added into 96-well plate. The 0 μM group was set as the control group. Cell viability was measured after 48 h. The CCK-8 (20 μL) was added to each well and incubated for 2 h in the dark at 37 °C. The absorbance of the solution was measured at 450 nm using Varioskan LUX (Thermo Fisher Scientific, Waltham, MA, USA). Cell viability was calculated based on the following formula: Viability = OD450 (treated group)/OD450 (control group). The IC₅₀ values were expressed as inhibited cell growth by 50%, analyzed by Graph Prism 7.0 (Graph Pad Saftware, La Jolla, CA, USA).