Tumor tissues were fixed in paraformaldehyde (4%) and embedded in paraffin. After cutting into 5‐μm thick sections, the sections were incubated with primary antibodies against KIAA0101 (ab226255; 1:500; Abcam), Ki‐67 (1:200; ab15580; Abcam), E‐cadherin (20874‐1‐AP; 1:1000; Rosemont) and N‐cadherin (22018‐1‐AP; 1:1000; Rosemont). Thereafter, the sections were incubated with corresponding secondary antibodies (1:4000; ab205718; Abcam). After staining with diaminoaniline (DAB; Beyotime) and counterstaining with hematoxylin (Beyotime), a microscope (Leica) was used to observe the immunohistochemistry images.
Diaminoaniline dab
Manufactured by Beyotime
Diaminoaniline (DAB) is a lab equipment product used as a chromogenic substrate in various applications. It is a chemical compound that can be used to visualize the presence of certain target analytes in biological samples through color development. The core function of DAB is to serve as a detection reagent, enabling researchers to identify and localize specific molecules or proteins of interest.
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Lab products found in correlation
3 protocols using diaminoaniline dab
1
Immunohistochemical Analysis of Tumor Tissues
2
Tumor Immunohistochemical Staining
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Dissected tumors were settled in 10% (v/v) formaldehyde, paraffin‐embedded, and slid into 4 μm sections. Next, the sections were hatched with FYN (1:500, ab227304, Abcam), Ki67 (1:200, ab15580, Abcam) and MMP9 (1:250, ab76003, Abcam) for 12 h at 4°C, and secondary antibody (1:4000, ab205718, Abcam) for 1 h. These sections were then stained with diaminoaniline (DAB; Beyotime) and counterstained with hematoxylin (Beyotime). Lastly, the images were obtained using a microscope (×200, Leica).
3
Immunohistochemical Analysis of TXNRD1
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Tumor tissues were fixed in formaldehyde (10%), embedded in paraffin, followed by cutting at a thickness of 4 μm slides. Next, these sections were incubated with the TXNRD1 (ab124954, 1:200, Abcam) antibody overnight at 4°C to measure TXNRD1 expression. After incubation with secondary antibody (ab150077, 1:1000, Abcam). After staining with diaminoaniline (DAB; Beyotime) and counterstaining with hematoxylin (Beyotime), the images were obtained under a microscope (Leica). The TXNRD1 score was defined as the percentage of TXNRD1-positive staining cells in the total number of cancer cells.
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