Collagen I matrices with a pore size ranging between 5 and 10 µm were reconstituted and characterized with already published protocols8 (link), 19 (link), 31 (link). Briefly, 13 mm coverslips (VWR International, Darmstadt, Germany) were cleaned, functionalized with 3-aminopropyltriethoxysilane (Alfa Aesar, Karlsruhe, Germany) to enable subsequent covalent binding of poly(styrene-alt-maleic anhydride) (PSMA; MW 30 000 g/mol, Sigma-Aldrich, Steinheim, Germany) monolayers. On top 3D collagen I matrices were reconstituted as previously described from rat tail collagen solutions (stock concentration 4.1 mg/ml, lot# 3298599, Corning, Amsterdam, Netherlands)8 (link). Fibrillogenesis took place in phosphate buffer at pH 7.5 with a collagen concentration of 2 mg/ml at 37 °C for 1 h. Collagen matrices were washed three times with PBS and equilibrated with cell culture medium overnight prior to cell seeding.
3 aminopropyltriethoxysilane
Manufactured by Thermo Fisher Scientific
Sourced in United States, United Kingdom
3-Aminopropyltriethoxysilane is a silane coupling agent used in various applications, including surface modification, adhesion promotion, and the preparation of functional materials. It contains three ethoxy groups and a primary amine group, which can react with different substrates to form covalent bonds. This product is commonly used in the manufacturing of silica-based materials, coatings, and composite materials.
Automatically generated - may contain errors
Lab products found in correlation
Tetraethyl orthosilicate, by Thermo Fisher Scientific (3 mentions)
Glutaraldehyde, by Merck Group (2 mentions)
Mpeg sva, by Laysan Bio (2 mentions)
Biotin peg sva, by Laysan Bio (2 mentions)
Tetraethyl orthosilicate, by Merck Group (2 mentions)
Ammonium persulfate, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Toluene, by Thermo Fisher Scientific (2 mentions)
N n dimethylformamide (dmf), by Thermo Fisher Scientific (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
13 mm coverslips, by Avantor (1 mentions)
Salicylaldehyde, by Merck Group (1 mentions)
Tetramethylammonium hydroxide, by Thermo Fisher Scientific (1 mentions)
Ethanol, by Chempur (1 mentions)
Acetonitrile, by Chempur (1 mentions)
Anhydrous erbium chloride, by Merck Group (1 mentions)
Methanol, by Chempur (1 mentions)
Hexadecyltrimethylammoniumchloride, by Tokyo Chemical Industry (1 mentions)
Absolute ethanol, by Merck Group (1 mentions)
Absolute methanol, by Merck Group (1 mentions)
15 protocols using 3 aminopropyltriethoxysilane
1
Reconstitution and Characterization of Collagen I Matrices
2
Hybrid Plastic Synthesis with Rare-Earth Complexes
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Trisilanolisobutyl-POSS (Hybrid Plastic,
USA), tetramethylammonium hydroxide (25% in mEthanol, Alfa Aesar),
and (3-aminopropyl)triethoxysilane (98%, Alfa Aesar) were purchased.
Salicylaldehyde (98%), anhydrous erbium chloride (99.9%), anhydrous
terbium chloride (99.9%), and anhydrous samarium chloride (99.9%)
were purchased from Sigma-Aldrich (Darmstadt, Germany) and used without
further purification. Ethanol (HPLC grade, J. T. Baker, Gdańsk,
Poland), mEthanol (HPLC grade, J. T. Baker, Gdańsk, Poland),
acetonitrile (HPLC grade, J. T. Baker, Gdańsk, Poland), toluene
(HPLC grade, J. T. Baker, Gdańsk, Poland), and tetrahydrofuran
(Chempur) were purchased. All solvents, except mEthanol and acetonitrile,
were used with further purification. Tetrahydrofuran and toluene were
purified by distillation with sodium wires, and Ethanol was purified
with metallic magnesium.
USA), tetramethylammonium hydroxide (25% in mEthanol, Alfa Aesar),
and (3-aminopropyl)triethoxysilane (98%, Alfa Aesar) were purchased.
Salicylaldehyde (98%), anhydrous erbium chloride (99.9%), anhydrous
terbium chloride (99.9%), and anhydrous samarium chloride (99.9%)
were purchased from Sigma-Aldrich (Darmstadt, Germany) and used without
further purification. Ethanol (HPLC grade, J. T. Baker, Gdańsk,
Poland), mEthanol (HPLC grade, J. T. Baker, Gdańsk, Poland),
acetonitrile (HPLC grade, J. T. Baker, Gdańsk, Poland), toluene
(HPLC grade, J. T. Baker, Gdańsk, Poland), and tetrahydrofuran
(Chempur) were purchased. All solvents, except mEthanol and acetonitrile,
were used with further purification. Tetrahydrofuran and toluene were
purified by distillation with sodium wires, and Ethanol was purified
with metallic magnesium.
3
Bioinspired Nanoparticle Synthesis Protocol
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Silver(I) nitrate (AgNO3) (99.9%), trisodium citrate dihydrate (C6H5Na3O7·2H2O, ≥99.5%), Adenosine monophosphate (99%), Gold(III) chloride trihydrate (HAuCl4·3H2O, 99.9%), sodium hydroxide (NaOH, BioXtra ≥ 98%, pellets, anhydrous), absolute ethanol (CH3CH2OH, ≥99.8%) and absolute methanol (CH3OH ≥ 99.9%) were obtained from Merck. Tannic acid (98%), Tetraethoxysilane (C8H20O4Si, 99+%), (3-Aminopropyl) triethoxysilane (C9H23NO3Si, 97%) were obtained from Alfa Aesar. Hexadecyltrimethylammonium Chloride (C19H42ClN, 95%) was obtained from TCI Chemicals. IROGRAN® A92P4637 was provided by Huntsman GmbH (Osnabruck, Germany). All reagents were used as received without further purification. Ultrapure water (type I) was used for the preparation of all the water-based solutions. The glassware was cleaned with aqua regia and rinsed with ultrapure water prior to the experiments.
4
Preparation and Characterization of Doxorubicin-Loaded Gelatin Nanoparticles
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Tetraethylorthosilicate (TEOS, 28%), N-Cetyltrimethylammonium bromide (CTAB) and (3-aminopropyl)triethoxysilane (APTES) were purchased from Alfa-Aesar. Gelatin, glutaraldehyde, N-hydroxysuccinimide (NHS), N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich. Doxorubicin (DOX) hydrochloride , sodium hyaluronate (HA) (MW = 200 kDa) was purchased from Aladdin. Heochst-33342 was purchased from Invitrogen Life Technologies Corporation. Other reagents were obtained from Xilong Reagent Company. Nanopure water (18.2 MΩ) was used in all experiments. All the chemicals were used as received without further purification.
5
Silanization and PEGylation of Coverslips
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Quartz coverslips were treated with 100% ethanol and 1 mM KOH. Then, aminosilanization of coverslips was carried out in a 1% (v/v) (3-Aminopropyl)triethoxy silane (Alfa Aesar, A10668, UK) solution in acetone. PEGylation was carried out by incubating mixture of biotinPEG-SVA and mPEG-SVA (Laysan Bio, AL) in the ratio of 1:10 prepared in 0.1 M NaHCO3 solution on the top of silanized coverslip for 3–4 hr. Finally, PEGylated coverslips were stored under dry nitrogen gas at −20°C.
6
Synthesis and Characterization of Curcumin-Loaded Silica Nanoparticles
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The following analytical-grade chemicals were purchased from Sigma-Aldrich (Israel) and were used as received: polyvinyl alcohol (PVA) white to off-white powder (Mw 89,000–98,000, 99+% hydrolyzed), curcumin, benzoic acid, 1,1′-carbonyldiimidazole, tetraethyl orthosilicate (TEOS) (99.999% purity), Trolox [(±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid] (97% purity) and 28% (v/v) aqueous NH4OH (99.99% purity). (3-Isocyanatopropyl)triethoxy silane, triethylamine, and (3-aminopropyl)-triethoxysilane were purchased from Alfa Aesar (Haverhill, MA, USA). Anhydrous tetrahydrofuran (THF) and anhydrous dichloromethane (DCM) were purchased from Acros Organics (Geel, Belgium). Acetone, n-hexane and ethanol absolute (EtOH) of analytical reagent (AR) grade were purchased from Gadot (Israel) and used without further purification.
7
Silanization and PEGylation of Coverslips
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Quartz coverslips were treated with 100% ethanol and 1 mM KOH. Then, aminosilanization of coverslips was carried out in a 1% v/v (3-Aminopropyl)triethoxy silane (Alfa Aesar, A10668, UK) solution in acetone. PEGylation was carried out by incubating a mixture of biotinPEG-SVA and mPEG-SVA (Laysan Bio, AL, USA) at a ratio of 1:20 prepared in 0.1 M NaHCO3 solution on the top of a silanized coverslip for at least 3-4 h. Finally, PEGylated coverslips were stored under dry nitrogen gas at -20 °C.
8
Functionalization of DeepTip Probes
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DeepTipTM probes (silicon nitride, nominal resonance frequency 67 kHz–17 kHz; k = 0.48–0.06 N/m) were produced and supplied by the company Bioactive Surfaces S.L. [38 ]. 3-Aminopropyltriethoxysilane, (purity 99%), and AcroSeal® was obtained from Acros Organics (Thermo Scientific Chemicals, Beerse, Geel, Belgium). Fluorescein 5(6)-isothiocyanate, 30% hydrogen peroxide solution, 50% glutaraldehyde solution in water, Streptavidin from Streptomyces avidinii, albumin biotinamidocaproyl-labeled bovine lyophilized powder, and poly(ethylene glycol)-(N-hydroxysuccinimide 5-pentanoate)-ether 2-(biotinylamino)ethane (MW = 10,000 Da, which corresponds to an approximate length for the spacer of 50 nm) were provided by Sigma-Aldrich (María de Molina, Madrid, Spain). An amount of 32% w/w ammonia solution was obtained from Scharlau (Sentmenat, Barcelona, Spain). Sodium dodecyl sulfate (SDS) was provided by Fisher Scientific (María de Molina, Madrid, Spain).
9
Fabrication and Functionalization of Polymer-Coated Glass Beads
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Glass beads SPHERIGLASS® A-Glass 2429 (70–100 μm diameter) obtained from Potters Industries LLC. Acrylic acid (AA), ammonium persulfate (APS), 1,2-bis(trimethoxysilyl)ethane, glutaraldehyde (GA), phosphate buffered saline (PBS) tablets, N-isopropylacrylamide (NIPAM), N,N′-methylene-bisacrylamide (BIS), N-tertbutylacrylamide (TBAm), N,N,N′,N′-tetramethyl-ethylenediamine (TEMED), trypsin from bovine pancreas, glucose, glycine, sorbitol, trehalose, 12-mercaptododecanoic acid, acetone, ethanol, methanol and toluene were purchased from Sigma-Aldrich, UK. 3-Aminopropyltriethoxysilane and sodium cyanoborohydride were obtained from Acros Organics. N-(3-Aminopropyl) methacrylamide hydrochloride (NAPMA) was purchased from PolySciences Inc., UK. Acetonitrile (ACN), sodium hydroxide (NaOH) and sulphuric acid (H2SO4) were obtained from Fisher Scientific (UK). Ultrapure water was produced by a Millipore Milli-Q system (Millipore, Bedford, MA, USA). Double-distilled ultrapure water (Millipore) was used for analysis. All chemicals and solvents were analytical or HPLC grade and were used without further purification. Phosphate buffered saline (PBS) was prepared as directed from PBS buffer tablets (Sigma-Aldrich, Gillingham, UK). N-Fluorescein acrylate was provided by Leicester Biotechnology Group.
10
Synthesis of Gold Nanoparticles Using Ascorbic Acid
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Hydrogen tetrachloroaurate (HAuCl4·3H2O, 99.999%, Aldrich), silver nitrate (AgNO3, 99%, Acros), ascorbic acid (AA, 99%, Merck), cetyltrimethylammonium bromide (CTAB, Aldrich, 98%), sodium borohydrate (NaBH4, 99%, Aldrich), hydrochloric acid (HCl, 37%, Merck), (3-aminopropyl)triethoxysilane (APTES, 99%, Acros), and sodium citrate (99%, Aldrich) were all used as received without further purification. The water used in the synthesis was of Milli-Q quality (18.2 MΩ cm), produced in a Simplicity 185 system (Millipore).
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