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Monoclonal anti tubulin e7

Monoclonal anti-tubulin (E7) is an antibody produced by the Developmental Studies Hybridoma Bank. It specifically recognizes and binds to the tubulin protein, which is a key structural component of the cytoskeleton in eukaryotic cells.

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2 protocols using monoclonal anti tubulin e7

1

Immunoblotting Analysis of Cellular Proteins

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SDS-PAGE and immunoblotting was performed using established protocols [41 (link)]. Nitrocellulose membranes were probed with anti-ATM (2C1, GeneTex, Irvine, CA), anti-RelA (sc-109, Santa Cruz Biotechnology, Santa Cruz, CA), anti-SOD2 (sc-30080, Santa Cruz Biotechnology) or monoclonal anti-tubulin (E7) obtained from the Developmental Studies Hybridoma Bank (Univ. of Iowa).
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2

Western Blot Analysis of Protein Markers

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Nitrocellulose membranes were probed with anti-TRIM29 (sc-33151, Santa Cruz Biotechnology), E-cadherin (sc-21791), EpCAM (sc-25308), N-cadherin (sc-59987), Vimentin (sc-32322), anti-Myc (#2276 Cell Signaling), anti-HA (#26183 Thermo Scientific), anti-TWIST (GTX12310), or monoclonal anti-tubulin (E7) (Developmental Studies Hybridoma Bank, Univ. of Iowa). Immunoblot signals were developed using chemiluminescence.
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