Procollagen type 1 peptide pip elisa kit

Dulbecco’s modified Eagle’s medium/F12 nutrient mixture Ham (DMEM/F12 3:1), antibiotic-antimycotic solution, trypsin-EDTA, phosphate buffered saline, Hank’s Balanced Salt Solution (HBSS) and fetal bovine serum (FBS) were purchased from Gibco (Grand Istand, NY, United States). Phosphoramidon disodium salt (Phosphoramidon), (+) sodium L-ascorbate (L-Ascorbate), epigallocatechin gallate (EGCG), N-succinyl-tri-alanyl-p-nitroanilide (STANA), albumin from bovine serum (BSA), Bradford reagent and trifluoroacetic acid (HPLC grade) were obtained from Sigma-Aldrich (St. Louis, MO, United States). Dimethyl sulfoxide (DMSO) was purchased from Amresco (Solon, OH, United States). Triton X-100 was obtained from Yakuri Pure Chemicals (Tokyo, Japan). Ethanol (HPLC grade) was obtained from Duksan Chem. Co. (Seoul, South Korea). Acetonitrile (HPLC grade) was purchased from Fisher Scientific Korea (Seoul, South Korea). Procollagen type I peptide (PIP) ELISA kit was obtained from Takara (Tokyo, Japan). Matrix Metalloproteinase-1 (MMP-1) human ELISA kit was purchased from Abcam (Cambridge, MA, United States). YOKUDELNA calibration kit was obtained from JEOL Ltd. (Tokyo, Japan).

Dulbecco’s modified Eagle’s medium/F12 nutrient mixture Ham (DMEM/F12 3:1), antibiotic–antimycotic solution, trypsin–EDTA, phosphate buffered saline, Hank’s Balanced Salt Solution (HBSS) and fetal bovine serum (FBS) were purchased from Gibco (Grand Istand, NY, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5 Diphenyl-2H-tetrazolium Bromide (MTT), phosphoramidon disodium salt (Phosphoramidon), ( +) sodium l-ascorbate (l-Ascorbate), epigallocatechin gallate (EGCG), N-succinyl-tri-alanyl-p-nitroanilide (STANA), albumin from bovine serum (BSA), Bradford reagent and trifluoroacetic acid (HPLC grade) were obtained in Sigma-Aldrich (St. Louis, MO, USA). Besides, dimethyl sulfoxide (DMSO) was purchased from Amresco (Solon, OH, USA). Triton X-100 was obtained from Yakuri Pure Chemicals (Tokyo, Japan). Ethanol (HPLC grade) was obtained from Duksan Chem. Co. (Seoul, South Korea). Acetonitrile (HPLC grade) was purchased from Fisher Scientific Korea (Seoul, South Korea). Procollagen type I peptide (PIP) ELISA kit was obtained from Takara (Tokyo, Japan). Matrix Metalloproteinase-1 (MMP-1) Human ELISA kit was purchased from Abcam (Cambridge, MA, USA). YOKUDELNA calibration kit was obtained from JEOL Ltd. (Tokyo, Japan).