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Penicillin streptomycin amphotericin

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Penicillin/streptomycin/amphotericin is a commonly used antibiotic and antifungal mixture for cell culture applications. It provides broad-spectrum antimicrobial protection against a variety of bacteria and fungi in cell culture environments.

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Lab products found in correlation

Glutamaxtm 1, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Raw264.7 mouse macrophage, by American Type Culture Collection (1 mentions)

2 protocols using penicillin streptomycin amphotericin

1

Cell Culture Maintenance Protocol

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HeLa and A549 were obtained from Cancer Research UK and human dermal fibroblast (Fdh) were obtained from ATCC and cultured in Dulbecco’s modified eagle medium (DMEM) GlutaMAXTM-1 supplemented with 10% fetal bovine serum (FBS) (Gibco, ThermoFisher Scientific) and 1% penicillin/streptomycin/amphotericin (Biowest). All cell lines were maintained at 37 °C in a humidified atmosphere at 5% CO2.
González-Pastor R., Lancelot A., Morcuende-Ventura V., San Anselmo M., Sierra T., Serrano J.L, & Martin-Duque P. (2021). Combination Chemotherapy with Cisplatin and Chloroquine: Effect of Encapsulation in Micelles Formed by Self-Assembling Hybrid Dendritic–Linear–Dendritic Block Copolymers. International Journal of Molecular Sciences, 22(10), 5223.
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2

Culturing RAW 264.7 Mouse Macrophages

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RAW 264.7 mouse macrophages (American Type Culture Collection, ATCC, Rockville, MD, USA) were grown in modified Dulbecco Eagle medium (DMEM) that was high in glucose (Biowest, Riverside, MO, USA) and supplemented with 10% fetal bovine serum (FBS) (v/v) and 1% penicillin/streptomycin/amphotericin (v/v) (Biowest). Cells were grown at 37 °C under constant conditions of humidity, 5% CO2, and 95% air.
Franca-Oliveira G., Martinez-Rodriguez A.J., Morato E, & Hernández-Ledesma B. (2023). Contribution of Proteins and Peptides to the Impact of a Soy Protein Isolate on Oxidative Stress and Inflammation-Associated Biomarkers in an Innate Immune Cell Model. Plants, 12(10), 2011.
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