E800 widefield microscope

Manufactured by Nikon

The Nikon E800 Widefield Microscope is a versatile laboratory instrument designed for a range of scientific applications. It features a wide field of view and high-quality optics to provide clear, detailed images of specimens. The E800 is capable of brightfield, darkfield, and phase contrast microscopy techniques.

Automatically generated - may contain errors

Lab products found in correlation

Fv1200 confocal microscope, by Olympus (1 mentions)

Close spelling variants of this product

E800 microscope (137 citations) Widefield microscope (11 citations) Nikon E800 widefield epifluorescence microscope (2 citations)

2 protocols using e800 widefield microscope

Immunohistochemical Staining of Mouse Liver

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse livers were fixed for 48 hours in 4% paraformaldehyde at 4°C, and then embedded in paraffin. Sections were cut and mounted on positively charged slides by American Histolabs (Gaithersburg, MD). Sections were stained with antibodies against VCAM-1 (No. MCA2297T, AbDSerotec, Kidlington, UK), CD31 (No. DIA 310, Dianova, Hamburg, Germany), as previously described [66 ]. Visualization was with diaminobenzidine (DAB) (No. D22187, Molecular Probes, Eugene, OR), which generates a brown-colored oxidation product upon reaction with the horseradish peroxidase (HRP) labeled secondary antibody. Development of the brown DAB color was monitored to prevent over saturation that would prevent quantification. A Nikon E800 Widefield Microscope was used for photography.

Dmitrieva N.I, & Burg M.B. (2015). Elevated Sodium and Dehydration Stimulate Inflammatory Signaling in Endothelial Cells and Promote Atherosclerosis. PLoS ONE, 10(6), e0128870.

+ Open protocol

+ Expand

Characterization of Fabricated Particles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Brightfield and fluorescent imaging were performed with the Nikon E-800 Widefield Microscope, with 40×, 60× in DIC mode and 100× objectives. The fabrication yield and spheroid ratio were quantified by counting the number of rods and spheres in each field of view and dividing the number of rods to the total number of the particles. The reported yield is the average of 3 batches ± standard error between different batches. The particle size, reported as the average ± standard deviation, was measured with NIS-Elements (AR 4.30.01) software for at least 100 particles per batch and averaged over three batches. Statistical analysis of particle size was done via two-way ANOVA test (PRISM software). SEM micrographs were obtained with JEOL-7800FLV FE-SEM. Samples for SEM were prepared by suspending particles in DI water and allowing these to be dried on a clean glass slide. Dried particle samples were sputter coated with gold before SEM imaging. Confocal images were obtained with Olympus FV 1200 Confocal Microscope.

Safari H., Adili R., Holinstat M, & Eniola-Adefeso O. (2018). Modified two-step emulsion solvent evaporation technique for fabricating biodegradable rod-shaped particles in the submicron size range. Journal of colloid and interface science, 518, 174-183.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!