Hbmsc growth medium

Manufactured by Cyagen

Sourced in China

HBMSC growth medium is a cell culture medium formulated to support the growth and expansion of human bone marrow-derived mesenchymal stem cells (hBMSCs) in vitro. The medium provides the necessary nutrients and growth factors to maintain hBMSC characteristics and promote cell proliferation.

Automatically generated - may contain errors

Lab products found in correlation

5 protocols using hbmsc growth medium

Osteogenic Differentiation of hBMSCs

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

hBMSCs were purchased from Cyagen Biosciences (Guangzhou, China). hBMSC growth medium were purchased from Cyagen Biosciences. Cells cultured in a atmosphere of 5% CO₂ at 37 °C. Osteogenic induction medium was prepared according to previous methods [49 (link)].
SERPINB2 recombinant protein was purchased from Amyjet Scientific (Abnova, wuhan, china). Antibodies used for western blotting including GAPDH (1:1000, Beyotime, shanghai, china), RUNX2 (1:1000, Beyotime), COL1A1 (1:1000, Beyotime), SERPINB2 (1:1000, Abcam), SP7/Osterix (1:1000, Abcam, shanghai, china), non-phosphorylated (active) β-catenin (1:1000; Cell Signaling Technology), or total β-catenin (1: 1000; Beyotime), phosphorylated and total ERK (1:1000; Cell Signaling Technology), phosphorylated and total AKT (1:1000; Cell Signaling Technology). Second antibodies Alexa Fluor 555 was purchased from Beyotime. Lipo6000 Transfection Reagents were purchased from Beyotime (Shanghai, china).
The siRNAs were purchased from Genepharma (Hangzhou, china).
hSERPINB2 sense: GCAAAGAAUCAAGUUGCAATT
hSERPINB2 antisense: UUGCAACUUGAUUCUUUGCTT
hNC sense: UUCUCCGAACGUGUCACGUTT
hNC antisense: TTAAGAGGCUUGCACAGUGCA
mSERPINB2 sense: GGAGAGAAGUCUGCAAGAUTT
mSERPINB2 antisense: AUCUUGCAGACUUCUCUCCTT
mNC sense: GGCUCUGTTCUUGUCACGUAA
mNC antisense: UUTTGAGGCAAGCACAGUGCA

Hang K., Ying L., Bai J., Wang Y., Kuang Z., Xue D, & Pan Z. (2021). Knockdown of SERPINB2 enhances the osteogenic differentiation of human bone marrow mesenchymal stem cells via activation of the Wnt/β-catenin signalling pathway. Stem Cell Research & Therapy, 12, 525.

+ Open protocol

+ Expand

hBMSC Culture and Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

hBMSCs were purchased from Cyagen Biosciences Inc. (HUXMA-01001, Guangzhou, China). Cells were incubated in hBMSC growth medium (Cyagen Biosciences, Guangzhou, China) at 37°C in a cell incubator containing 5% CO₂ with the medium being replaced every 3 days. Cells from passages three to seven were used in subsequent experiments.
Antibodies used for Western blotting including RUNX2 (ab236639, 1 : 1,000, Abcam), COL1A1 (AF1840, 1 : 1,000, Beyotime), SP7 (ab209484, 1 : 1,000, Abcam), YTHDF1 (ab252346, 1 : 1,000, Abcam), p62/SQSTM1 (AF5312, 1 : 1,000, Beyotime), LC3B (ab192890, 1 : 1,000, Abcam), active-β-Catenin (ab246504, 1 : 1,000, Abcam), and GAPDH (AF1186, 1 : 1,000, Beyotime). The small-interfering RNA- (siRNA-) mate transfection reagents were purchased from GenePharma (Shanghai, China).

Gao X., Wang J., Wang Y., Li W, & Pan Z. (2023). The m6A Reader YTHDF1 Accelerates the Osteogenesis of Bone Marrow Mesenchymal Stem Cells Partly via Activation of the Autophagy Signaling Pathway. Stem Cells International, 2023, 5563568.

+ Open protocol

+ Expand

Isolation of Healthy Human Bone Marrow Stromal Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

hBMSCs were isolated from the whole bone marrow of two healthy donors (two men, aged 20 and 22 years) using the method described previously (Zhang et al., 2018 (link)). All donors provided informed consent before collection of their bone marrow and the protocol was approved by the Ethics Committee of the Second Affiliate Hospital of Zhejiang University. All of the treatment procedures conformed to the ethical standards of the Declaration of Helsinki. Adherent hBMSCs were then cultured in culture flasks with hBMSC growth medium (Cyagen Biosciences, Guangzhou, China) consisting of human bone marrow stromal cells basal medium, 10% human bone marrow stromal cell-qualified fetal bovine serum, 1% penicillin-streptomycin and 1% glutamine in an incubator at 37°C with 5% CO₂, with passage after reaching 80% confluence. Cells from passages 3–5 were used in subsequent experiments.

Bai J., Xu J., Hang K., Kuang Z., Ying L., Zhou C., Ni L., Wang Y, & Xue D. (2021). Glycyrrhizic Acid Promotes Osteogenic Differentiation of Human Bone Marrow Stromal Cells by Activating the Wnt/β-Catenin Signaling Pathway. Frontiers in Pharmacology, 12, 607635.

+ Open protocol

+ Expand

Gelatin-based hydrogel for hBMSC culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

GA (purity, > 97.5%, as determined by high-performance liquid chromatography [HPLC]) was purchased from TargetMol (Wellesley Hills, MA, USA). The GA was dissolved in dimethyl sulfoxide (DMSO) and stored at -20°C in the dark. Photopolymerisable gelatine methacryloyl (GelMA) hydrogels were purchased from Engineering For Life (Suzhou, China). XAV-939, an inhibitor of the Wnt/β-catenin signalling pathway, was purchased from Selleck Chemicals (Houston, TX, USA). We used 10 μM XAV-939 based on a previous study (Iwatani et al., 2017) .
Cell isolation and culture hBMSCs were isolated from the whole bone marrow of two healthy donors (two men, aged 20 and 22 years) using the method described previously (Zhang et al., 2018) . All donors provided informed consent before collection of their bone marrow and the protocol was approved by the Ethics Committee of the Second Affiliate Hospital of Zhejiang University. All of the treatment procedures conformed to the ethical standards of the Declaration of Helsinki. Adherent hBMSCs were then cultured in culture flasks with hBMSC growth medium (Cyagen Biosciences, Guangzhou, China) in an incubator at 37°C with 5% CO 2 , with passage after reaching 80% confluence. Cells from passages 3-5 were used in subsequent experiments.

Xue D., Bai J., Xu J., Hang K., Kuang Z., Zhou C., Li Y., & Wang Y. (2020). Glycyrrhizic acid promotes osteogenic differentiation of human bone mesenchymal stem cells by activating the Wnt/β-catenin signalling pathway.

+ Open protocol

+ Expand

Osteogenic Differentiation of hBMSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

The hBMSCs provided by Cyagen Biosciences (HUXMA-01001; Guangzhou, China) had the potential to differentiate into osteoblasts, chondrocytes, and adipocytes under appropriate conditions. Adherent hBMSCs were cultured in asks with hBMSC growth medium (Cyagen Biosciences, Guangzhou, China) in an incubator at 37°C under 5% CO2 and were passaged after reaching 80% con uence. The medium was replaced every 3 days; cells from passages 3-5 was used in subsequent experiments.
Recombinant human MFG-E8 (rhMFG-E8) protein was purchased from R&D Systems (Minneapolis, MN). Primary antibodies against GAPDH (CST#5174), COL1A1 (CST#72026), GSK3β (CST#12456), and phospho-GSK-3β (Ser9) (CST#5558) were purchased from Cell Signaling Technology (Danvers, MA).
Primary antibodies against RUNX2 (ab192256), active β-catenin (ab246504), and total β-catenin (ab223075) were obtained from Abcam (Cambridge, UK). AR-A014418, a competitive and selective ATP inhibitor of GSK3β, was purchased from Selleck Chemicals (Houston, TX).

Bai J., Zhang W., Hang K., Zhao G., Zhong H., Zhou C., Xu J., Zhang W., Chen E., Wu J., Liu L., & Xue D. (2021). MFG-E8, A Novel Target of Promoting Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!