Human NPC cell lines CNE1 and CNE2 were purchased from American Type Culture Collection (ATCC), Manassas, VA, USA and maintained in Roswell Park Memorial Institute-1640 medium containing 10% fetal bovine serum with penicillin (100 U/mL) and streptomycin (100 U/mL) at 37°C with 5% CO2. Primary antibodies against Skp2, tubulin and the secondary antibodies were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Anti-p21 and anti-p57 antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Lipofectamine 2000 was purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) and curcumin (CAS number 458-37-7, 99.5% purity) was obtained from Sigma-Aldrich Co. (St Louis, MO, USA). Cells were treated with 0.1% dimethyl sulfoxide (DMSO) for control conditions.
Curcumin cas number 458 37 7
Manufactured by Merck Group
Sourced in United States
Curcumin (CAS number 458-37-7) is a naturally occurring compound extracted from the rhizome of the turmeric plant (Curcuma longa). It is a yellow-colored polyphenol with a chemical formula of C21H20O6. Curcumin is used as a laboratory reagent and has various applications in research and scientific experiments.
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Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Penicillin, by American Type Culture Collection (1 mentions)
Streptomycin, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)
2 protocols using curcumin cas number 458 37 7
1
Investigating Skp2 and p21/p57 in NPC Cells
2
Colorectal Cancer Cell Line Culture
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The colorectal cancer HCT-116 cell line was purchased from the Iranian national cell bank (Pasteur institute, Tehran, Iran). The cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% (v/v) fetal bovine serum and 1% (v/v) penicillin–streptomycin solution (both from GIBCO, USA) at 37 °C in a humidified environment with 5% CO2. Curcumin (CAS Number 458-37-7; Sigma-Aldrich, USA) and mPEG urethane gemini surfactant nanoparticles were a kind gift from the Institute for Color Science and Technology, Tehran, Iran.
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