Rabbit anti pgc 1α

For preparing total lysates, cells were first washed with and then scraped into cold PBS supplemented with a proteinase inhibitor cocktail. After centrifugation, cell pellets were lysed in RIPA buffer (50 mm Tris/HCl, 150 mm NaCl, 0.1% SDS, 1% NP‐40, and proteinase inhibitor; pH 7.4). Total lysates (30 µg protein/well) were separated on a 10% SDS/polyacrylamide gel and then processed for immunoblotting. The primary antibodies including mouse anti‐LRH‐1 (Abcam, Cambridge, UK; ab41901), rabbit anti‐LGR5 (Abcam; ab75850), mouse anti‐HIF‐1α (Abcam; ab1), rabbit anti‐ALDH‐1 (Genetex, Irvine, CA, USA; GTX123973), mouse anti‐OXPHOS cocktail (Abcam; ab110413), rabbit anti‐PGC‐1α (Santa Cruz, Dallas, CA, USA; sc‐13067), mouse anti‐β‐actin (Merck Millipore, Burlington, MA, USA; MAB1501), rabbit anti‐GAPDH (Genetex; GTX100118) were properly diluted and then incubated with the blots at 4 °C overnight, followed by incubation with the horseradish peroxidase‐conjugated secondary antibodies.
Finally, signals were detected using an enhanced chemiluminescence system (ECL, NEN Life Science, Boston, MA, USA) and their intensities were quantified by densitometry (imagej, Betheada, MD, USA).