Clone ep618y

Tumors were fixed in 10% formalin for at least 24 h and then embedded in paraffin. Both the original patient tumor and mouse-grown tumor tissues were sectioned at 5-μm thickness and stained with hematoxylin and eosin (H&E). Immunohistochemistry was performed to examine the expression levels of p53 and DPC4 in the original patient tumors and in the tumors grown in the PDOX and PDHX models following the protocol of the Department of Diagnostic Pathology at the Asan Medical Center as previously described [44 (link)]. In brief, after deparaffinization and antigen retrieval, the slides were labeled with a monoclonal antibody against p53 (clone DO-7, 1:3,000; Dako, Glostrup, Denmark) and DPC4 (clone EP618Y, 1:100; GeneTex, Irvine, CA, USA). Labeling was detected using the avidin-biotin complex staining method. 3,3′-Diaminobenzidine was used as the chromogen for p53, and 3-amino-9-ethylcarbazole was used for detecting DPC4. A pathologist who was experienced in pancreatic cancer reviewed the slides to compare the tumor architecture and desmoplastic appearance in the patient and PDX models.

Tumors were fixed in 10% formalin for at least 24hand then embedded in paraffin. Both human and mouse tumor tissues were sectioned at a 5μm thickness and stained with H&E. Immunohistochemistry(IHC) was performed to examine the expression of p53 and DPC4 in the primary human tumors, as previously described [27 (link)], following the protocol of the Department of Diagnostic Pathology at the Asan Medical Center. Briefly, after deparaffinization and antigenic retrieval, the slides were labeled with a monoclonal antibody against p53 (cloneDO-7, 1:3,000; DAKO, Glostrup, Denmark) and DPC4 (clone EP618Y, 1:100; GeneTex, Irvine, CA, USA). Labeling was detected using the avidin-biotin complex staining method. 3, 3′-diaminobenzidine (DAB) was used as the chromogen for p53and 3-amino-9-ethylcarbazole was used for DPC4. A pathologist who was experienced in pancreatic cancer reviewed the slides to compare the tumor architecture and desmoplastic appearance.