Phospho y1054 1055 tyk2

The following antibodies were purchased from Cell Signaling Technology (Boston, MA, USA): phospho‐EGFR (#3777), EGFR (#4267), phospho‐InsR/IGF1R (#3024), IGF1R (#3018), phospho‐Y705‐STAT3 (# 9145), STAT3 (#9139), STAT1 (#9172), phospho‐ Y1007/1008‐JAK2 (#3776), JAK2 (#3230), phospho‐ Y1022/1023‐JAK1 ( #3331), JAK1 (#3332), phospho‐ Y1054/1055‐TYK2 (#9321), TYK2 (#9312), phospho‐ S32‐Ikbα (#2859), Ikbα (#4814), phospho‐S176/180‐IKKα/β (#2697), IKKα (#2682), IKKβ (#8943), NF‐κB p65(#8242), phospho‐S536‐NF‐κB p65(#3033). The antibodies for gp130 (#sc‐656) and α‐Tubulin (#SC‐5286) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The antibody for β‐Actin was purchased from Abmart (#P30002M). The antibody for phospho‐Y1230/1231‐VEGFR3 (#CY1115) was purchased from Cell Applications. Secondary HRP‐conjugated antibodies were purchased from Multi Sciences Biotech (Hangzhou, China). The anti‐Flag affinity gel was purchased from Bimake (#B23101, Shanghai, China).

The following antibodies were from Cell Signaling Technology (Boston, MA, USA): phospho-EGFR (1:2000, #3777), EGFR (1:2000, #4267), phospho-InsR/IGF1R (1:2000, #3024), IGF1R (1:2000, #3018), phospho-Y705-STAT3 (1:3000, #9145), STAT3 (1:3000, #9139), STAT1 (1:2000, #9172), phospho-Y1007/1008-JAK2 (1:2000, #3776), JAK2 (1:2000, #3230), phospho-Y1022/1023-JAK1 (1:1000, #3331), JAK1 (1:2000, #3332), phospho-Y1054/1055-TYK2 (1:1000, #9321), TYK2 (1:2000, #9312), and Cofilin (1:2000, #5175). The antibodies for GP130 (1:1000, #sc-656) and α-Tubulin (1:3000, #SC-5286) were from Santa Cruz Biotechnology (Dallas, TX, USA). The antibodies for β-Actin were from Abmart (1:3000, #P30002M). The antibodies for phospho-Y1230/1231-VEGFR3 (1:2000, #CY1115) were from Cell Applications. Secondary HRP-conjugated antibodies were from Multi Sciences Biotech (1:5000, Hangzhou, China). Anti-Flag affinity gel was purchased from Bimake (1:50, #B23101, Shanghai, China).