Biotin mouse anti human igg

Manufactured by BD

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Biotin mouse anti-human IgG is a laboratory reagent used for the detection and analysis of human immunoglobulin G (IgG) in various research and diagnostic applications. It consists of a biotinylated mouse-derived antibody that specifically binds to human IgG molecules.

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Prism 9.0 for mac os, by GraphPad (1 mentions)

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Biotin anti-human IgG (2 citations) Mouse anti (2 citations) Biotin-conjugated mouse anti-human IgG mAb (2 citations)

2 protocols using biotin mouse anti human igg

IgG ELISA Quantification Protocol

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High binding ELISA plates were coated with recombinant Rv2626c at 2.5 μg/mL or different dilutions of a human IgG antibody standard (calibration curve; Vigam Liquid Inmunoglobulina Humana Normal). Plates were blocked with 2% skim milk in saline solution. Samples (plasma obtained from heparinized non-stimulated whole blood) were diluted 1:100 and run in duplicate. Then, a secondary antibody (biotin mouse anti-human IgG - BD Pharmingen, USA) was added. Plates were consequently incubated for 1 h at 37 °C. High sensitivity streptavidin - horseradish peroxidase enzyme (Thermo Fisher Scientific, USA) was incorporated and 3, 3′5, 5′-tetramethylbenzidine (TMB) (Sigma-Aldrich, USA) substrate was added. Finally, sulfuric acid was incorporated to stop the enzyme reaction and plates were read at 450 nm. The results are expressed as ng/ml of IgG.

Amiano N.O., Morelli M.P., Pellegrini J.M., Tateosian N.L., Rolandelli A., Seery V., Castello F.A., Gallego C., Armitano R., Stupka J., Erschen M.A., Ciallella L.M., de Casado G.C., Cusmano L., Palmero D.J., Iovanna J.L, & García V.E. (2020). IFN-γ and IgG responses to Mycobacterium tuberculosis latency antigen Rv2626c differentiate remote from recent tuberculosis infection. Scientific Reports, 10, 7472.

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Omicron Spike RBD Antibody ELISA Protocol

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Nunc 96-well immune ELISA plates were coated with 1 μg/ml of B.1.1.529 (Omicron) Spike RBD protein in carbonate buffer (Sigma Aldrich) for 2 hours at 37°C before washing with PBS (0.05% Tween) (PBST) and blocking at 37°C for 1 hour with PBS containing 1% Bovine Serum Albumin (BSA). Plates were washed in PBST again before application of 50 μl of diluted sera to each well. All serum dilutions were run in duplicate and a four-point dilution series was run for each sample. Following overnight incubation at 4°C, plates were washed with PBST and wells incubated with 1:1000 dilution of Biotin Mouse Anti-human IgG (BD Pharmingen, 555785) at room temperature for 1 hour. Plates were washed again before application of 1:200 dilution of Streptavidin Horseradish Peroxidase (HRP) (Bio-techne, DY998) for 30 min followed by a final wash and then assay development using 3,3′, 5,5;-tetramethylbenzidine (TMB) substrate (Sigma Aldrich, T0440). Color development was stopped after 5 min by the addition of 0.18M H₂SO₄ and OD450nm values for each well measured using a FLUOstar® Omega Plate Reader. Analysis of ELISA data was performed in Prism 9.0 for Mac OS (GraphPad). Data for serial dilutions were plotted and area under the curve calculated for each individual serum sample.

Reynolds C.J., Pade C., Gibbons J.M., Otter A.D., Lin K.M., Muñoz Sandoval D., Pieper F.P., Butler D.K., Liu S., Joy G., Forooghi N., Treibel T.A., Manisty C., Moon J.C., Semper A., Brooks T., McKnight Á., Altmann D.M., Boyton R.J., Abbass H., Abiodun A., Alfarih M., Alldis Z., Altmann D.M., Amin O.E., Andiapen M., Artico J., Augusto J.B., Baca G.L., Bailey S.N., Bhuva A.N., Boulter A., Bowles R., Boyton R.J., Bracken O.V., O’Brien B., Brooks T., Bullock N., Butler D.K., Captur G., Carr O., Champion N., Chan C., Chandran A., Coleman T., Couto de Sousa J., Couto-Parada X., Cross E., Cutino-Moguel T., D’Arcangelo S., Davies R.H., Douglas B., Di Genova C., Dieobi-Anene K., Diniz M.O., Ellis A., Feehan K., Finlay M., Fontana M., Forooghi N., Francis S., Gibbons J.M., Gillespie D., Gilroy D., Hamblin M., Harker G., Hemingway G., Hewson J., Heywood W., Hickling L.M., Hicks B., Hingorani A.D., Howes L., Itua I., Jardim V., Lee W.Y., Jensen M., Jones J., Jones M., Joy G., Kapil V., Kelly C., Kurdi H., Lambourne J., Lin K.M., Liu S., Lloyd A., Louth S., Maini M.K., Mandadapu V., Manisty C., McKnight Á., Menacho K., Mfuko C., Mills K., Millward S., Mitchelmore O., Moon C., Moon J., Muñoz Sandoval D., Murray S.M., Noursadeghi M., Otter A., Pade C., Palma S., Parker R., Patel K., Pawarova M., Petersen S.E., Piniera B., Pieper F.P., Rannigan L., Rapala A., Reynolds C.J., Richards A., Robathan M., Rosenheim J., Rowe C., Royds M., Sackville West J., Sambile G., Schmidt N.M., Selman H., Semper A., Seraphim A., Simion M., Smit A., Sugimoto M., Swadling L., Taylor S., Temperton N., Thomas S., Thornton G.D., Treibel T.A., Tucker A., Varghese A., Veerapen J., Vijayakumar M., Warner T., Welch S., White H., Wodehouse T., Wynne L., Zahedi D., Chain B, & Moon J.C. (2022). Immune boosting by B.1.1.529 (Omicron) depends on previous SARS-CoV-2 exposure. Science (New York, N.y.).

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