D luciferin potassium

Three weeks after rAAV9-GFP-empty injection, the mice were subjected to live imaging observation to determine whether the adeno-associated virus successfully infected the liver.
The mice were anesthetized and injected intraperitoneally with D-Luciferin potassium (Meilunbio, China). Ten minutes later, the mice were placed in the imaging chamber of the Xenogen in vivo imaging system. X-rays were used to further determine the specific location of the adeno-associated virus infection.

DSPE-PEG-2000 was obtained from Meilun Biotechnology Co., Ltd (Dalian, China). P(TMZ)n was synthesized from Beixin Biotechnology Co., Ltd (Suzhou, China). 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) and 1,2-dioleoyl-3-trimethylammoniumpropane (DOTAP) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). AnnexinV-fluorescein isothiocyanate (FITC)/propidium iodide (PI) was obtained from Nanjing Key GEN BioTECH Co. Ltd (Nanjing, China). Calcein/PI Live/Dead Viability/Cytotoxicity Assay Kit were purchased from Beyotime Biotechnology. Angiopep-2 (TFFYGGSRGKRNNF KTEEY) was purchased from GLBiochem Ltd (Shanghai, China). Cell Counting Kit-8 (CCK8) and D-Luciferin potassium were purchased from Meilun Biotechnology Co., Ltd (Dalian, China). LysoTracker™ green was brought from Invitrogen (Carlsbad, CA, USA). MGMT Rabbit monoclonal antibody (mAb) was got from Cell Signaling Technology Co., Ltd (Danvers, MA, USA). Beta-actin mAb was bought from Protein tech Antibodies People Trust (Chicago, IL, USA). Cy5-labeled siRNA (Cy5-siRNA), negative control siRNA with a scrambled sequence (nonsense, antisense strand,5′-UCGAAGUACUCAGCGUAAGdTdT-3′), and siRNA targeting MGMT mRNA (siMGMT, antisense strand, 5′-UGUCGCUCAAACAUCCAUCdTdT-3′) were purchased from Beixin Biotechnology Co., Ltd (Suzhou, China).