Pt3 ef1a bmi1

Deguelin (>97% purity) and other chemical reagents, including Tris, NaCl, SDS, and DMSO, for molecular biology and buffer preparation, were purchased from Sigma‐Aldrich (St. Louis, MO, USA). z‐VAD‐fmk (cat#S7023), Necrostatin‐1 (cat#S8037), and GSK'872 (cat#S8465) were purchased from Selleckchem (Houston, TX, USA). Lentivirus plasmids containing pLKO.1‐shBmi1 (#1, TRCN0000020154; #2, TRCN0000020155; #3, TRCN0000020156; #4, TRCN0000020157; #5, TRCN0000020158) were purchased from Thermo Scientific (Rockford, IL, USA), pLKO.1‐shNoxa (V3SH11240‐224893462) was purchased from GE Dharmacon (Lafayette, CO, USA). The Bmi1 expression construct pT3‐EF1a‐Bmi1 (#31783), the luciferase reporter pGL3‐Noxa‐N1 (#26112), pLKO.1‐shGFP (#30323), the lentiviral packaging plasmid psPAX2 (#12260), and the envelope plasmid pMD2.G (#12259) were available on Addgene (Cambridge, MA, USA). The pGL3‐Basic and the Renilla luciferase reporter construct pRL‐SV40 (Promega, Madison, WI, USA) was used as previously described.43