This study was a randomized post-test only control group design in vitro experimental research. The HeLa cells were randomly divide into 8 groups, with Group 1 (negative control), 2 (was given EPI ½ IC50), 3 (was given EPI IC50), 4 (was given EPI 2IC50), 5 (was given DOX IC50), G6 (was given EPI ½ IC50 + DOX ½IC50), 7 (was given IC50 CIS), and 8 (was given EPI ½ IC50 + CIS ½IC50). The EPI compound was synthesized at the Faculty of Mathematics and Natural Sciences of Universitas Gadjah Mada by Dr. Andi Hairil Alimuddin. HeLa cells obtained from Parasitology Laboratory, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Indonesia. The cells were grown using RPMI (Gibco), supplemented with 10% Fetal Bovine Serum (FBS Qualified, Gibco, Invitrogen USA), 1.5% (w/w) penicillin-streptomycin (Gibco, Invitrogen USA), and 0.5% (w/w) fungizone (Gibco, Invitrogen USA). Cells were incubated at 37°C and 5% CO2.
Fetal bovine serum fbs qualified
Manufactured by Thermo Fisher Scientific
Sourced in United States
Fetal Bovine Serum (FBS) Qualified is a laboratory reagent used to supplement cell culture media. It provides essential growth factors, nutrients, and other components to support the growth and maintenance of a variety of cell types in vitro.
Automatically generated - may contain errors
2 protocols using fetal bovine serum fbs qualified
1
Cytotoxicity of Epirubicin and Combination Treatments on HeLa Cells
2
Phytochemical Screening and Cell Culture
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The phytochemical screening of extract of flavonoid test used HCl concentrated with Mg powder. The saponin was tested by adding water in the extract in reaction tubes.
Alkaloid tests used Dragendorf and Mayer reagents. The tannin test was performed by adding FeCl 3 to filtrate extracts. Meanwhile, steroid and triterpenoid tests used Liebermann-Burchard.
Cell Line Culture MCF-7 cells were cultivated in Dulbecco's Modified Eagle Media (DMEM) (Gibco), supplemented with 10% (v/v) Fetal Bovine Serum (FBS) Qualified (Gibco, Invitrogen USA), 2% (v/v) penicillin-streptomycin (Gibco, Invitrogen USA), and 0,5% fungizone (Gibco, Invitrogen USA). Cells culture was incubated at temperature 37 o C and 5% CO 2 .
Alkaloid tests used Dragendorf and Mayer reagents. The tannin test was performed by adding FeCl 3 to filtrate extracts. Meanwhile, steroid and triterpenoid tests used Liebermann-Burchard.
Cell Line Culture MCF-7 cells were cultivated in Dulbecco's Modified Eagle Media (DMEM) (Gibco), supplemented with 10% (v/v) Fetal Bovine Serum (FBS) Qualified (Gibco, Invitrogen USA), 2% (v/v) penicillin-streptomycin (Gibco, Invitrogen USA), and 0,5% fungizone (Gibco, Invitrogen USA). Cells culture was incubated at temperature 37 o C and 5% CO 2 .
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