Bio plex pro mouse cytokine grp 1 panel 23 plex kit

The Bio-Plex Pro Mouse Cytokine Grp I Panel 23-plex Kit (Bio-Rad; #M60009RDPD) was used to determine the serum inflammatory factors in mice, including IL-1α, IL-4, IL-6 and TNF- α.

Mouse blood samples were centrifuged at 4,500 × g for 10 min to obtain the serum, which was diluted 1:4 with the dilution solution. The diluted solution (50 μL) was tested using a Bio-Plex Pro Mouse Cytokine Grp I Panel 23-plex kit (Bio-Rad, Austin, TX, USA) at Wayne Biotechnology (Shanghai) Co., Ltd. according to a previous report (19 (link)) with a bead suspension platform (Bio-Plex MAGPIX System, Bio-Rad). Considering the circadian rhythm of melatonin secretion, we collected serum samples at 4 am (GMT+8). Mouse melatonin levels were measured using enzyme-linked immunosorbent assay kits (ELISA; Yuanxin Biotechnology Co., Ltd., Shanghai, China), and T-tau, interleukin (IL)-6, MCP-1, tumor necrosis factor (TNF)-α, and LCN2 levels were also measured using ELISA kits (Longdeng Biotechnology Co., Ltd., Shanghai, China). The uric acid serum level was tested using the uric acid assay kit (Nanjing Jiancheng Biological Engineering Research Institute, China).