Zorbax c18 reversed phase column

Manufactured by Agilent Technologies

Sourced in Canada

The Zorbax C18 reversed-phase column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of organic compounds. The column features a chemically bonded C18 stationary phase, which provides efficient and reliable separation of analytes based on their hydrophobic interactions with the stationary phase.

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Lab products found in correlation

Agilent hp 1100 series, by Agilent Technologies (1 mentions) 6460 lc ms ms system, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Zorbax C18 column C18 ZORBAX C18 column

2 protocols using zorbax c18 reversed phase column

Synthesis and Purification of Isotope-Labeled pHLIP

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All the isotope-labelled pHLIP (NH₂-GGEQNPIYWARYADWLFTTPLLLLDLALLVDADEGT-CO₂H, with labelled sites shown in bold) were synthesized manually using the routine 9-fluorenylmethyloxycarbonyl (Fmoc) protecting group chemistry with preloaded Fmoc-Thr(tBu)-Wang resin (0.3 mmol g⁻¹ substitution, AAPPTec Inc., Louisville, KY). The isotope-labelled amino acids were purchased from Cambridge Isotope Laboratory, Inc. (Tewksbury, MA), and Fmoc protection of the free amino group was accomplished using literature approaches52 (link). The crude peptides were purified using high-performance liquid chromatography (Agilent HP 1100 series, Agilent Technologies Inc., Santa Clare, CA) installed with a Zorbax C18 reversed-phase column (Agilent Technologies Inc., Santa Clare, CA). A linear gradient with water and acetonitrile was utilized for the purification. The final products were verified with matrix-assisted laser desorption/ionization–time of flight mass spectrometry (Mass Spectrometer Facility, University of Illinois).

Shu N.S., Chung M.S., Yao L., An M, & Qiang W. (2015). Residue-specific structures and membrane locations of pH-low insertion peptide by solid-state nuclear magnetic resonance. Nature Communications, 6, 7787.

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LC-MS/MS Analysis of Enzyme Activity

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An Agilent 6460 LC-MS/MS system was used. An Agilent Zorbax C18 reversed-phase column (50 mm × 2.1 mm internal diameter, 1.8-μm particle size) was maintained at 30 °C. Mobile phase A consisted of 5% acetonitrile with 0.1% formic acid and mobile phase B consisted of 1:1 methanol: acetonitrile with 0.1% formic acid. The LC conditions and MS parameters are described in the Supplemental Material file that accompanies the online version of this article at http://www.clinchem.org/content/vol63/issue4. The extracted ion chromatograms of P and IS of 6 enzymes including GAA can be viewed in online Supplemental Fig. 1.

Lin N., Huang J., Violante S., Orsini J.J., Caggana M., Hughes E.E., Stevens C., DiAntonio L., Liao H.C., Hong X., Ghomashchi F., Kumar A.B., Zhou H., Kornreich R., Wasserstein M., Gelb M.H, & Yu C. (2017). Liquid Chromatography-Tandem Mass Spectrometry Assay of Leukocyte Acid α-Glucosidase for Post-Newborn Screening Evaluation of Pompe Disease. Clinical chemistry, 63(4), 842-851.

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