Foetal bovine serum (fbs)

Manufactured by Yeasen

Sourced in China, United States

Foetal bovine serum is a cell culture media supplement derived from the blood of bovine fetuses. It provides a rich source of nutrients, growth factors, and other components necessary for the growth and maintenance of various cell types in vitro.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Hemin, by Merck Group (1 mentions) Dulbecco modified eagle medium (dmem), by Solarbio (1 mentions) Baf a1, by MedChemExpress (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)

3 protocols using foetal bovine serum (fbs)

Cellular Responses to Hemin, Baf-A1, and ZnPP

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BV‐2 and HT22 were obtained from the China Center for Type Culture Collection and cultured in DMEM (11995; Solarbio Co., Ltd.) containing 10% foetal bovine serum (40130ES76; Yeasen Co., Ltd.), supplemented in acceptable condition at 37°C, 5% CO₂. Cells were seeded at 5 × 10⁴ cells/mL. When the cells were confluent to 70%, hemin (H9039; Sigma‐Aldrich Co., Ltd.), Baf‐A1 (HY‐100558; MedChemExpress Co., Ltd.) and ZnPP (HY‐101193; MedChemExpress Co., Ltd.) were used in this experiment.

Wang R., Liang Z., Xue X., Mei H., Ji L., Wang B., Chen W., Gao C., Yuan S., Wu T., Qi H., Hu S., Yi L., Song Y., Liao R, & Chen B. (2023). Microglial FoxO3a deficiency ameliorates ferroptosis‐induced brain injury of intracerebral haemorrhage via regulating autophagy and heme oxygenase‐1. Journal of Cellular and Molecular Medicine, 28(1), e18007.

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Culturing Eca-109 Esophageal Cancer Cells

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Eca-109 (human ESCC cell line) was obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and was cultured in RPMI 1640 medium (Gibco, Thermo Fisher Scientific™, USA) supplemented with 10% foetal bovine serum (YEASEN, Shanghai, China) and 100 U/mL penicillin/streptomycin (Gibco, Thermo Fisher Scientific™, USA) in 5% CO₂ incubator at 37 °C.

Ying J., Huang H.H., Zhang M.M, & Chen J.F. (2021). Up-regulation of SOCS4 promotes cell proliferation and migration in esophageal squamous cell carcinoma. Translational Cancer Research, 10(5), 2416-2427.

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Culturing ESCC cell lines under hypoxia

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The Eca109, Kyse150, TE-1 and TE-10 human ESCC cell lines were confirmed by cell morphology and genomic short tandem repeats. All cell lines were incubated in RPMI-1640 with 10% foetal bovine serum (Yeasen Biotech Co., Ltd.) and 1% penicillin-streptomycin (Invitrogen, USA) at 37°C in a humidified atmosphere with 5% CO₂. In the hypoxic experiments, the cells were treated with 150 µM CoCl₂ for 24 h at 37°C and then cultured in a humidified atmosphere with 5% CO₂ at 37°C.

Yi H., Han Y., Li Q., Lin R., Zhang J., Yang Y., Wang X, & Zhang L. (2023). Prognostic impact of the combination of HIF‑1α and GLUT1 in patients with oesophageal squamous cell carcinoma. Oncology Letters, 26(3), 404.

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